Detailed information of TA system TA172314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 325615..326095
• Replicon: chromosome
• Accession: NZ_CP061298
• Organism: Polynucleobacter paludilacus strain MWH-Mekk-C3

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: AOC06_RS01805
• Protein ID: WP_215380761.1
• Coordinates: 325826..326095 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: AOC06_RS01800
• Protein ID: WP_215380759.1
• Coordinates: 325615..325842 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOC06_RS01775 (AOC06_01775)	321551..322543	+	993	WP_215380745.1	NAD-dependent epimerase/dehydratase family protein	-
AOC06_RS01780 (AOC06_01780)	322587..323606	-	1020	WP_215380748.1	tyrosine-type recombinase/integrase	-
AOC06_RS01785 (AOC06_01785)	323688..323930	+	243	WP_215380751.1	XRE family transcriptional regulator	-
AOC06_RS01790 (AOC06_01790)	323927..324376	+	450	WP_215380754.1	hypothetical protein	-
AOC06_RS01795 (AOC06_01795)	324486..325415	+	930	WP_215380757.1	hypothetical protein	-
AOC06_RS01800 (AOC06_01800)	325615..325842	+	228	WP_215380759.1	ribbon-helix-helix domain-containing protein	Antitoxin
AOC06_RS01805 (AOC06_01805)	325826..326095	+	270	WP_215380761.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOC06_RS01810 (AOC06_01810)	326371..326712	+	342	WP_206603336.1	DUF6516 family protein	-
AOC06_RS01815 (AOC06_01815)	326709..327083	+	375	WP_215380763.1	MarR family transcriptional regulator	-
AOC06_RS01820 (AOC06_01820)	327147..327524	+	378	WP_112294899.1	hypothetical protein	-
AOC06_RS01825 (AOC06_01825)	327578..328648	-	1071	WP_215380765.1	glycosyltransferase	-
AOC06_RS01830 (AOC06_01830)	328720..329739	-	1020	WP_112294897.1	ionic transporter y4hA	-
AOC06_RS01835 (AOC06_01835)	329810..330172	+	363	WP_215284471.1	CbiX/SirB N-terminal domain-containing protein	-
AOC06_RS01840 (AOC06_01840)	330095..330907	-	813	WP_215284657.1	uroporphyrinogen-III C-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (3-35)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10445.28 Da
• Isoelectric Point: 10.1284

>T172314 WP_215380761.1 NZ_CP061298:325826-326095 [Polynucleobacter paludilacus]
MAWIIKYTESSSKQLKKLDKQTALRVLDYMDERVAVLNDPRSLGKNLKGPKIGEYWRYRVGDIRVICNIVDGQMMVLVIE
IGNRREVYR

Nucleotide

• Length: 270 bp

>T172314 NZ_CP079725:845182-845592 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8577.69 Da
• Isoelectric Point: 4.4857

>AT172314 WP_215380759.1 NZ_CP061298:325615-325842 [Polynucleobacter paludilacus]
MAVSIRLDPEIEQRLDQLAAQTGRAKSYYLRELIEGGLDDLEDFYLADAVMERVRRGSEKLLDATQVRKDLGLDH

Nucleotide

• Length: 228 bp

>AT172314 NZ_CP079725:844935-845201 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA