Detailed information of TA system TA172312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 597157..597740
• Replicon: chromosome
• Accession: NZ_CP061297
• Organism: Polynucleobacter asymbioticus strain MWH-MoIso1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AOC19_RS03095
• Protein ID: WP_215377490.1
• Coordinates: 597399..597740 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AOC19_RS03090
• Protein ID: WP_215377488.1
• Coordinates: 597157..597399 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOC19_RS03080 (AOC19_03085)	592312..594114	+	1803	WP_215377484.1	NAD(P)H-dependent oxidoreductase subunit E	-
AOC19_RS03085 (AOC19_03090)	594148..597042	+	2895	WP_215377486.1	formate dehydrogenase subunit alpha	-
AOC19_RS03090 (AOC19_03095)	597157..597399	+	243	WP_215377488.1	PbsX family transcriptional regulator	Antitoxin
AOC19_RS03095 (AOC19_03100)	597399..597740	+	342	WP_215377490.1	endoribonuclease MazF	Toxin
AOC19_RS03105 (AOC19_03110)	598020..599681	+	1662	WP_215377494.1	L-aspartate oxidase	-
AOC19_RS03110 (AOC19_03115)	599720..599866	+	147	WP_215377496.1	hypothetical protein	-
AOC19_RS03115 (AOC19_03120)	599878..600732	-	855	WP_215377497.1	carboxylating nicotinate-nucleotide diphosphorylase	-
AOC19_RS03120 (AOC19_03125)	600745..601872	-	1128	WP_215377499.1	quinolinate synthase NadA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-109)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12468.56 Da
• Isoelectric Point: 9.7237

>T172312 WP_215377490.1 NZ_CP061297:597399-597740 [Polynucleobacter asymbioticus]
MAKTYIPNAGDIVWLEFDPQAGHEQAGRRPAVVLTPEAYNKKTNLMICCPLTTKIKGYPFEVLVEIDGIQSAILSDQVKS
LDWKTRKAKYKGAVSTFALAEVRSKAKALLSIT

Nucleotide

• Length: 342 bp

>T172312 NZ_CP079725:344237-344605 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8985.47 Da
• Isoelectric Point: 8.6592

>AT172312 WP_215377488.1 NZ_CP061297:597157-597399 [Polynucleobacter asymbioticus]
MQTIIKKWGNSPALRLNTAVMKSAHLNIDQIVSVKVQRGRIVIEPIVQKEYQLDDLLAGITAKNIHHENDFGEPVGKELL

Nucleotide

• Length: 243 bp

>AT172312 NZ_CP079725:344019-344240 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA