Detailed information of TA system TA172309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 1089908..1090455
• Replicon: chromosome
• Accession: NZ_CP061296
• Organism: Polynucleobacter sp. MWH-P3-07-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ICU98_RS05700
• Protein ID: WP_251365311.1
• Coordinates: 1089908..1090150 (+)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ICU98_RS05705
• Protein ID: WP_215335826.1
• Coordinates: 1090147..1090455 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICU98_RS05665 (ICU98_05660)	1084918..1085178	+	261	WP_215351165.1	PbsX family transcriptional regulator	-
ICU98_RS05670 (ICU98_05665)	1085178..1085546	+	369	WP_215351168.1	type II toxin-antitoxin system PemK/MazF family toxin	-
ICU98_RS05675 (ICU98_05670)	1085564..1086949	+	1386	WP_215351171.1	phosphomannomutase/phosphoglucomutase	-
ICU98_RS05680 (ICU98_05675)	1087019..1087276	+	258	WP_251365310.1	type II toxin-antitoxin system CcdA family antitoxin	-
ICU98_RS05685 (ICU98_05680)	1087276..1087593	+	318	WP_215351173.1	CcdB family protein	-
ICU98_RS05690 (ICU98_05685)	1087784..1088911	+	1128	WP_215351176.1	quinolinate synthase NadA	-
ICU98_RS05695 (ICU98_05690)	1088917..1089771	+	855	WP_215351179.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ICU98_RS05700 (ICU98_05695)	1089908..1090150	+	243	WP_251365311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICU98_RS05705 (ICU98_05700)	1090147..1090455	+	309	WP_215335826.1	putative addiction module antidote protein	Antitoxin
ICU98_RS05710 (ICU98_05705)	1090515..1091900	-	1386	WP_215351181.1	hypothetical protein	-
ICU98_RS05715 (ICU98_05710)	1091941..1093599	-	1659	WP_215351183.1	L-aspartate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-76)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 9144.78 Da
• Isoelectric Point: 10.6611

>T172309 WP_251365311.1 NZ_CP061296:1089908-1090150 [Polynucleobacter sp. MWH-P3-07-1]
MGRAKIQARIKRLVEGNPGNTKSVGGKVFEMKIDFGPGYRIYYTKYEDVMYLLLIGGNKRTQSKDIQIAKYLAKNIQAMR

Nucleotide

• Length: 243 bp

>T172309 NZ_CP079719:495264-495614 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10945.76 Da
• Isoelectric Point: 9.5075

>AT172309 WP_215335826.1 NZ_CP061296:1090147-1090455 [Polynucleobacter sp. MWH-P3-07-1]
MKKTIISPYDVVEHLRTPKEMALYLQACIEESDGDAAFIAKALGDIARAKGMAQVAKESGLSRESLYKALSGDRIPGFDT
VLKVLAALGLSLHTKPTSKARA

Nucleotide

• Length: 309 bp

>AT172309 NZ_CP079719:494977-495258 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA