Detailed information of TA system TA172304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 279490..280095
• Replicon: chromosome
• Accession: NZ_CP061295
• Organism: Polynucleobacter sp. MWH-S4W17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C2755_RS01525
• Protein ID: WP_215321465.1
• Coordinates: 279490..279792 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C2755_RS01530
• Protein ID: WP_215321466.1
• Coordinates: 279793..280095 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2755_RS01495 (C2755_01495)	275449..276270	-	822	WP_215321460.1	energy transducer TonB	-
C2755_RS01500 (C2755_01500)	276294..276713	-	420	WP_072583297.1	protein TolR	-
C2755_RS01505 (C2755_01505)	276737..277393	-	657	WP_215321461.1	protein TolQ	-
C2755_RS01510 (C2755_01510)	277470..278714	+	1245	WP_215321462.1	serine hydroxymethyltransferase	-
C2755_RS01515 (C2755_01515)	278754..279200	+	447	WP_215321463.1	transcriptional regulator NrdR	-
C2755_RS01520 (C2755_01520)	279204..279344	-	141	WP_215321464.1	hypothetical protein	-
C2755_RS01525 (C2755_01525)	279490..279792	+	303	WP_215321465.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2755_RS01530 (C2755_01530)	279793..280095	+	303	WP_215321466.1	putative addiction module antidote protein	Antitoxin
C2755_RS01535 (C2755_01535)	280154..280819	-	666	WP_215321467.1	adenylate kinase	-
C2755_RS01540 (C2755_01540)	280944..281708	-	765	WP_215321468.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
C2755_RS01545 (C2755_01545)	281730..281909	-	180	WP_215321469.1	Trm112 family protein	-
C2755_RS01550 (C2755_01550)	281982..283073	-	1092	WP_215321470.1	tetraacyldisaccharide 4'-kinase	-
C2755_RS01555 (C2755_01555)	283100..283564	-	465	WP_215321471.1	biopolymer transporter ExbD	-
C2755_RS01560 (C2755_01560)	283561..284211	-	651	WP_215321472.1	MotA/TolQ/ExbB proton channel family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-95)

Antitoxin

• Uncharacterized protein HI1420 (12-99)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11485.40 Da
• Isoelectric Point: 10.3357

>T172304 WP_215321465.1 NZ_CP061295:279490-279792 [Polynucleobacter sp. MWH-S4W17]
MYEIDKTVEFELWLSSIRDPLTRGRLLARLRRVALGNLGDVASISESNGIWEMREHFGAGWRMYYLRRGRVVILMLGGGS
KATQNADIRKVKQMLLAMED

Nucleotide

• Length: 303 bp

>T172304 NZ_CP079716:c5686873-5686583 [Pseudomonas amygdali]
ATGACCTATAACCTCGAATTCGATGCGCGGGCTCTAAAGGAATGGCACAAATTAGGGGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCCAATCGTCTGCATGCATTGCCTGACTGCTACAAAATCA
AACTGCGCAGTAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCTGTGGACAAG
CGTGAGCGAGAGGAGGTTTACCGTAAGGCTGCTGATCGGTTGGGCGGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11033.98 Da
• Isoelectric Point: 10.3342

>AT172304 WP_215321466.1 NZ_CP061295:279793-280095 [Polynucleobacter sp. MWH-S4W17]
MKKTKKIKIKDISKFDVVDYLKTDADIAGYLTAVLEDGDPALFVAAIGDIARAKGMSDIAKKSGVTRESLYRALKIEARP
RFETVTRVIHALGMRLTVHA

Nucleotide

• Length: 303 bp

>AT172304 NZ_CP079716:c5687102-5686863 [Pseudomonas amygdali]
ATGGCGTCTATCAACGTTCGTATCGACGACGATCTCAAAGCGCGTGCTTACCTCGAGCTGGAAAAGCTTGGCGTCACCCC
CTCGGAGTTGCTGCGCCAAACCCTGCAGTACGTAGCTGAGCGTGGTCAGCTGCCCTTCAAGACGGTTCTGCTGACAGAGG
AAGACGAAGCGCTGTTGGCAACTGTGCGCGAGCGCCTTGCAGCTCCGCAGCGGGTCAAGGTTTCACTGGATGACCTATAA