Detailed information of TA system TA172299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 355306..355828
• Replicon: chromosome
• Accession: NZ_CP061294
• Organism: Polynucleobacter sp. MWH-Spelu-300-X4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ICV01_RS01835
• Protein ID: WP_215288045.1
• Coordinates: 355544..355828 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ICV01_RS01830
• Protein ID: WP_215288044.1
• Coordinates: 355306..355554 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICV01_RS01810 (ICV01_01810)	350664..351674	+	1011	WP_215288036.1	hypothetical protein	-
ICV01_RS01815 (ICV01_01815)	351668..352399	+	732	WP_215288038.1	hypothetical protein	-
ICV01_RS01820 (ICV01_01820)	352410..353822	-	1413	WP_215288040.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
ICV01_RS01825 (ICV01_01825)	354202..354999	+	798	WP_215288042.1	transglycosylase SLT domain-containing protein	-
ICV01_RS01830 (ICV01_01830)	355306..355554	+	249	WP_215288044.1	plasmid stabilization protein	Antitoxin
ICV01_RS01835 (ICV01_01835)	355544..355828	+	285	WP_215288045.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICV01_RS01840 (ICV01_01840)	355917..356405	-	489	WP_215288048.1	transcription antitermination factor NusB	-
ICV01_RS01845 (ICV01_01845)	356414..356905	-	492	WP_215288050.1	6,7-dimethyl-8-ribityllumazine synthase	-
ICV01_RS01850 (ICV01_01850)	356925..358058	-	1134	WP_215288052.1	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	-
ICV01_RS01855 (ICV01_01855)	358158..358766	-	609	WP_215288054.1	riboflavin synthase	-
ICV01_RS01860 (ICV01_01860)	358770..359921	-	1152	WP_215288055.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
ICV01_RS01865 (ICV01_01865)	359949..360548	-	600	WP_215288057.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11251.28 Da
• Isoelectric Point: 10.6689

>T172299 WP_215288045.1 NZ_CP061294:355544-355828 [Polynucleobacter sp. MWH-Spelu-300-X4]
MTYKLDFYEDALKEWRKLDGTVREQFRIKLKDRLENPRVPAAKLRDAPNRYKIKLKSVGYRLVYEVLDKEIVVVVVAIGK
RNRNEIYRIAAKRL

Nucleotide

• Length: 285 bp

>T172299 NZ_CP079716:c4397421-4397239 [Pseudomonas amygdali]
GTGCAGAGCAGGCAATTGATAAAGGAGCTTGAAGCAGATGGATGGATTCTGGATCGTGTTTCAGGCAGTCACCATATGTT
CAAGCATCCTGAAAAACTTCAGACAGTCCCCGTGCCGCATCCCAAGAAAGACTTGCCATTCGGAACGGTGCGGGCCATCA
AAAAGCTTGCCGGGCTGGTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9028.37 Da
• Isoelectric Point: 5.0491

>AT172299 WP_215288044.1 NZ_CP061294:355306-355554 [Polynucleobacter sp. MWH-Spelu-300-X4]
MAHAILSGTTASVTELKKDPMNTVAAGEGFPVAILNRNEPVFYCIPTKAYEDLLDRLEDLELNALANARKGQKRIRVTLD
DL

Nucleotide

• Length: 249 bp

>AT172299 NZ_CP079716:c4397203-4396799 [Pseudomonas amygdali]
ATGAAATATCCAATGTGCATAGAATGGGGAAGCGAAACCACGGCGTTTGGTATCCAGATTCCCGATATCCCAGGTGCCGT
AACCGCAGGCGATACGTTCGAGGAAGCCCACGCTGCGGCGGTGGAGATTGCTCATATCATGCTTCAGGAAATAGCAGCTG
GTGGTGGAACCATACCCAAGGCCGGCACTGTTGCCGAGCACGCCAGGAATGCCGATTTCGCCGGAATGGGCTGGGGCATG
ATCGAGATTGACGTAACGCCTTATCTTGGTAAGACGGAGAAGGTCAACGTCACACTGCCTGGTTTCGTGATCAAGCAGAT
CGATCGCTATGTACGGGATCACAGCATCAAGAGTCGTTCCACCTTCCTGGCCGACGCGGCGCTGGAAAAACTGGGCCGGG
CATGA