Detailed information of TA system TA172281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1301435..1302043
• Replicon: chromosome
• Accession: NZ_CP061285
• Organism: Polynucleobacter sp. MWH-UH35A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ICV36_RS06780
• Protein ID: WP_215399952.1
• Coordinates: 1301747..1302043 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ICV36_RS06775
• Protein ID: WP_215399951.1
• Coordinates: 1301435..1301746 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICV36_RS06745 (ICV36_06740)	1296439..1296858	-	420	WP_215399945.1	MarR family winged helix-turn-helix transcriptional regulator	-
ICV36_RS06750 (ICV36_06745)	1297067..1298242	-	1176	WP_215399946.1	MFS transporter	-
ICV36_RS06755 (ICV36_06750)	1298381..1299103	-	723	WP_215399947.1	tetratricopeptide repeat protein	-
ICV36_RS06760 (ICV36_06755)	1299252..1299686	+	435	WP_215399948.1	hypothetical protein	-
ICV36_RS06765 (ICV36_06760)	1299696..1299878	+	183	WP_215399949.1	hypothetical protein	-
ICV36_RS06770 (ICV36_06765)	1300092..1301132	-	1041	WP_215399950.1	tyrosine-type recombinase/integrase	-
ICV36_RS06775 (ICV36_06770)	1301435..1301746	-	312	WP_215399951.1	putative addiction module antidote protein	Antitoxin
ICV36_RS06780 (ICV36_06775)	1301747..1302043	-	297	WP_215399952.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICV36_RS06785 (ICV36_06780)	1302191..1303894	-	1704	WP_215399953.1	amidohydrolase	-
ICV36_RS06790 (ICV36_06785)	1304081..1304506	-	426	WP_215399954.1	BLUF domain-containing protein	-
ICV36_RS06795 (ICV36_06790)	1304513..1305304	-	792	WP_215399955.1	methylmalonyl-CoA decarboxylase	-
ICV36_RS06800 (ICV36_06795)	1305319..1305534	-	216	WP_215399956.1	hypothetical protein	-
ICV36_RS06805 (ICV36_06800)	1305552..1306769	-	1218	WP_215399957.1	polyhydroxyalkanoate depolymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (17-100)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11085.98 Da
• Isoelectric Point: 10.5610

>T172281 WP_215399952.1 NZ_CP061285:c1302043-1301747 [Polynucleobacter sp. MWH-UH35A]
MFKVLETDNFREWLGGIKDLSTRIRLARRLDRARLGNLGDVKALADGVYEMREKFGPGWRMYYVLRGKTIIVVLGGGDKS
SQVSDIKAAKKLAKLLED

Nucleotide

• Length: 297 bp

>T172281 NZ_CP079713:1989268-1989486 [Salmonella enterica subsp. arizonae]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAACTGTCCGATAATGAGTTAGCTGTATTTTACTCAGCGGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTTTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11282.26 Da
• Isoelectric Point: 10.5220

>AT172281 WP_215399951.1 NZ_CP061285:c1301746-1301435 [Polynucleobacter sp. MWH-UH35A]
MKTVKVEKKIKVSDLAEFDMTRHLKSKRAMTEYLNQVIADGDDAELAAALGHIARAKGMAEISKASGITREALYKALKPN
TKPRFDTVSKVLGALGMRITIHA

Nucleotide

• Length: 312 bp

>AT172281 NZ_CP079713:1988866-1989240 [Salmonella enterica subsp. arizonae]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTTGGTTAATGACCCAACCTCAGCAGTCAATCTCCAGCTCAATGAGCTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAGATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTCAATCGGGTAACCGACT
GTTTCGCTGTTTCGTCAACGCCACAAGGGCCAACCCTGTTAGTTTGTCTTGTTAA