Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 572144..572780 Replicon chromosome
Accession NZ_CP061284
Organism Bacillus halotolerans strain XH-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ICJ61_RS02955 Protein ID WP_003156187.1
Coordinates 572430..572780 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ICJ61_RS02950 Protein ID WP_003225183.1
Coordinates 572144..572425 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICJ61_RS02930 568499..569098 - 600 WP_024120294.1 rhomboid family intramembrane serine protease -
ICJ61_RS02935 569193..569558 + 366 WP_106020948.1 holo-ACP synthase -
ICJ61_RS02940 569724..570740 + 1017 WP_106021112.1 outer membrane lipoprotein carrier protein LolA -
ICJ61_RS02945 570857..572026 + 1170 WP_024120297.1 alanine racemase -
ICJ61_RS02950 572144..572425 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ICJ61_RS02955 572430..572780 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ICJ61_RS02960 572896..573720 + 825 WP_081638235.1 RsbT co-antagonist protein RsbRA -
ICJ61_RS02965 573725..574090 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ICJ61_RS02970 574093..574494 + 402 WP_106020946.1 serine/threonine-protein kinase RsbT -
ICJ61_RS02975 574506..575513 + 1008 WP_024120300.1 phosphoserine phosphatase RsbU -
ICJ61_RS02980 575574..575903 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
ICJ61_RS02985 575900..576382 + 483 WP_024120302.1 anti-sigma B factor RsbW -
ICJ61_RS02990 576348..577136 + 789 WP_024120303.1 RNA polymerase sigma factor SigB -
ICJ61_RS02995 577136..577735 + 600 WP_024120304.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T172272 WP_003156187.1 NZ_CP061284:572430-572780 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T172272 NZ_CP079712:1588972-1589841 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACACCCCCGTCCCGAACCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGTGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCTTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGTCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT172272 WP_003225183.1 NZ_CP061284:572144-572425 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT172272 NZ_CP079712:1589834-1590667 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTTTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References