Detailed information of TA system TA172265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 75651..76186
• Replicon: plasmid pDA61218-116
• Accession: NZ_CP061207
• Organism: Escherichia coli strain DA61218

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0M1UJC2
• Locus tag: ICI53_RS24750
• Protein ID: WP_001270422.1
• Coordinates: 75899..76186 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: ICI53_RS24745
• Protein ID: WP_001132895.1
• Coordinates: 75651..75902 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI53_RS24715	70668..70979	-	312	WP_001309256.1	hypothetical protein	-
ICI53_RS24720	71043..71402	-	360	WP_001336695.1	DUF1281 domain-containing protein	-
ICI53_RS24725	71409..71552	-	144	WP_000270807.1	DUF1281 domain-containing protein	-
ICI53_RS24730	71719..72495	-	777	Protein_95	hypothetical protein	-
ICI53_RS24735	72729..73691	-	963	WP_000756328.1	ParB/RepB/Spo0J family partition protein	-
ICI53_RS24740	73688..74893	-	1206	WP_000817635.1	AAA family ATPase	-
ICI53_RS24745	75651..75902	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ICI53_RS24750	75899..76186	+	288	WP_001270422.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICI53_RS24755	76188..76599	+	412	Protein_100	DDE-type integrase/transposase/recombinase	-
ICI53_RS24760	77243..78220	+	978	WP_001309253.1	RepB family plasmid replication initiator protein	-
ICI53_RS24765	78463..79203	-	741	WP_001066947.1	tyrosine-type recombinase/integrase	-
ICI53_RS24770	79324..79512	-	189	WP_001332784.1	hypothetical protein	-
ICI53_RS24775	79879..81048	+	1170	WP_001072358.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-48 / sul1 / qacE / ant(3'')-Ia	senB	1..116466	116466
	-	flank	IS/Tn	-	-	71932..72072	140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11262.30 Da
• Isoelectric Point: 10.4703

>T172265 WP_001270422.1 NZ_CP061207:75899-76186 [Escherichia coli]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T172265 NZ_CP079692:c4802439-4801906 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGATTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT172265 WP_001132895.1 NZ_CP061207:75651-75902 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT172265 NZ_CP079692:c4802716-4802450 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0M1UJC2

Antitoxin

• AlphaFold DB: L4J0V6