Detailed information of TA system TA172239

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2164082..2164873
• Replicon: chromosome
• Accession: NZ_CP061206
• Organism: Escherichia coli strain DA61218

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: ICI53_RS10075
• Protein ID: WP_000691791.1
• Coordinates: 2164082..2164507 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: ICI53_RS10080
• Protein ID: WP_000939437.1
• Coordinates: 2164538..2164873 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI53_RS10050	2159748..2160245	-	498	WP_000509808.1	hypothetical protein	-
ICI53_RS10055	2160927..2161313	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
ICI53_RS10060	2161936..2162667	-	732	WP_001216639.1	TIGR02646 family protein	-
ICI53_RS10065	2162664..2162885	-	222	WP_125112077.1	hypothetical protein	-
ICI53_RS10070	2162870..2164061	-	1192	Protein_1951	AAA family ATPase	-
ICI53_RS10075	2164082..2164507	-	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
ICI53_RS10080	2164538..2164873	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ICI53_RS10085	2164873..2165346	-	474	WP_001292742.1	DNA repair protein RadC	-
ICI53_RS10090	2165376..2166190	-	815	Protein_1955	DUF945 domain-containing protein	-
ICI53_RS10095	2166426..2167379	-	954	WP_000290406.1	hypothetical protein	-
ICI53_RS10100	2167972..2168586	+	615	WP_000772910.1	inovirus Gp2 family protein	-
ICI53_RS10105	2168704..2168922	+	219	WP_000070772.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2161936..2185867	23931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T172239 WP_000691791.1 NZ_CP061206:c2164507-2164082 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T172239 NZ_CP079673:2194046-2194429 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT172239 WP_000939437.1 NZ_CP061206:c2164873-2164538 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT172239 NZ_CP079673:2193804-2194046 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6