Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1136464..1137014 Replicon chromosome
Accession NZ_CP061206
Organism Escherichia coli strain DA61218

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag ICI53_RS05345 Protein ID WP_001160966.1
Coordinates 1136464..1136778 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ICI53_RS05350 Protein ID WP_000125566.1
Coordinates 1136781..1137014 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICI53_RS05320 1132139..1133425 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
ICI53_RS05325 1133425..1134414 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ICI53_RS05330 1134411..1135232 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ICI53_RS05335 1135235..1135612 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ICI53_RS05340 1135619..1136467 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ICI53_RS05345 1136464..1136778 - 315 WP_001160966.1 CcdB family protein Toxin
ICI53_RS05350 1136781..1137014 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ICI53_RS05355 1137100..1137579 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
ICI53_RS05360 1137771..1139633 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
ICI53_RS05365 1139626..1140156 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ICI53_RS05370 1140264..1141595 - 1332 WP_001183207.1 MFS transporter -
ICI53_RS05375 1141654..1141941 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T172228 WP_001160966.1 NZ_CP061206:c1136778-1136464 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T172228 NZ_CP079668:c4786327-4785794 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT172228 WP_000125566.1 NZ_CP061206:c1137014-1136781 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT172228 NZ_CP079668:c4786604-4786338 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References