Detailed information of TA system TA172220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 25345..25915
• Replicon: plasmid ps12201A.p5
• Accession: NZ_CP061200
• Organism: Piscirickettsia salmonis strain Ps12201A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ICC15_RS19060
• Protein ID: WP_129556708.1
• Coordinates: 25345..25680 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ICC15_RS19065
• Protein ID: WP_016212365.1
• Coordinates: 25673..25915 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICC15_RS19025	20538..21317	+	780	WP_081377351.1	transposase	-
ICC15_RS19030	21506..21838	+	333	WP_032126739.1	transposase	-
ICC15_RS19035	21910..22269	+	360	WP_188322496.1	IS630 transposase-related protein	-
ICC15_RS19040	22232..22798	+	567	WP_155046745.1	IS630 family transposase	-
ICC15_RS19045	22964..23341	+	378	WP_016212154.1	DDE-type integrase/transposase/recombinase	-
ICC15_RS19050	23648..24031	-	384	WP_016212152.1	hypothetical protein	-
ICC15_RS19055	24501..25229	-	729	WP_054300202.1	IS6 family transposase	-
ICC15_RS19060	25345..25680	-	336	WP_129556708.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ICC15_RS19065	25673..25915	-	243	WP_016212365.1	antidote-toxin recognition MazE family protein	Antitoxin
ICC15_RS19070	26124..27097	+	974	Protein_36	IS30 family transposase	-
ICC15_RS19075	27225..27665	+	441	Protein_37	tyrosine-type recombinase/integrase	-
ICC15_RS19080	27733..28460	+	728	Protein_38	IS6 family transposase	-
ICC15_RS19085	28743..30428	+	1686	WP_075275141.1	protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36773	36773
	-	inside	IScluster/Tn	-	-	62..32167	32105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12345.36 Da
• Isoelectric Point: 10.3188

>T172220 WP_129556708.1 NZ_CP061200:c25680-25345 [Piscirickettsia salmonis]
MNKVPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVEIPKNNSVHGAVLVNQLTTK
DFRARGWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 336 bp

>T172220 NZ_CP079658:c7299-6883 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.09 Da
• Isoelectric Point: 4.4750

>AT172220 WP_016212365.1 NZ_CP061200:c25915-25673 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT172220 NZ_CP079658:c7526-7296 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGAGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA