Detailed information of TA system TA172219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 70972..71615
• Replicon: plasmid ps12201A.p2
• Accession: NZ_CP061192
• Organism: Piscirickettsia salmonis strain Ps12201A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: ICC15_RS17410
• Protein ID: WP_016210974.1
• Coordinates: 71208..71615 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: ICC15_RS17405
• Protein ID: WP_016210972.1
• Coordinates: 70972..71199 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICC15_RS17350	66068..66373	+	306	WP_016210958.1	hypothetical protein	-
ICC15_RS17355	66527..66826	+	300	WP_016210976.1	hypothetical protein	-
ICC15_RS17360	66873..67688	+	816	WP_016210962.1	hypothetical protein	-
ICC15_RS17365	67711..68271	+	561	WP_016210973.1	hypothetical protein	-
ICC15_RS17370	68240..68602	+	363	WP_016210959.1	hypothetical protein	-
ICC15_RS17375	68595..68942	+	348	WP_016210966.1	hypothetical protein	-
ICC15_RS17380	68939..69178	+	240	WP_016210963.1	hypothetical protein	-
ICC15_RS17385	69274..69561	+	288	WP_036794065.1	hypothetical protein	-
ICC15_RS17390	69558..69842	+	285	WP_036794070.1	hypothetical protein	-
ICC15_RS17395	69844..70317	+	474	WP_016210960.1	single-stranded DNA-binding protein	-
ICC15_RS17400	70432..70824	+	393	WP_129556726.1	hypothetical protein	-
ICC15_RS17405	70972..71199	+	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ICC15_RS17410	71208..71615	+	408	WP_016210974.1	DUF4411 family protein	Toxin
ICC15_RS17415	71732..72412	+	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
ICC15_RS17420	72591..72884	+	294	WP_016210977.1	zinc ribbon domain-containing protein	-
ICC15_RS17425	73102..73284	-	183	WP_129556724.1	hypothetical protein	-
ICC15_RS17430	73429..73608	-	180	WP_155046773.1	phosphatase	-
ICC15_RS17435	73655..74020	+	366	WP_016212235.1	hypothetical protein	-
ICC15_RS17440	74321..74704	+	384	WP_032126134.1	HNH endonuclease	-
ICC15_RS17445	74792..75271	+	480	WP_016212234.1	P27 family phage terminase small subunit	-
ICC15_RS17450	75275..75484	+	210	WP_016212231.1	hypothetical protein	-
ICC15_RS17455	75539..75856	+	318	WP_164997282.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89447	89447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T172219 WP_016210974.1 NZ_CP061192:71208-71615 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T172219 NZ_CP079656:c14256-13906 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT172219 WP_016210972.1 NZ_CP061192:70972-71199 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT172219 NZ_CP079656:c13901-13599 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5