Detailed information of TA system TA172205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 84084..85707
• Replicon: plasmid pLD67-1-157kb
• Accession: NZ_CP061187
• Organism: Escherichia coli strain LD67-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: IB283_RS24440
• Protein ID: WP_000088046.1
• Coordinates: 84382..85707 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: IB283_RS24435
• Protein ID: WP_024136327.1
• Coordinates: 84084..84377 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB283_RS24410	79956..81197	+	1242	WP_001053338.1	VWA domain-containing protein	-
IB283_RS24415	81760..82041	-	282	WP_000077926.1	hypothetical protein	-
IB283_RS24420	82091..82282	-	192	WP_000398480.1	hypothetical protein	-
IB283_RS24425	82374..82715	-	342	WP_001151575.1	hypothetical protein	-
IB283_RS24430	83088..83480	+	393	WP_000341066.1	hypothetical protein	-
IB283_RS24435	84084..84377	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
IB283_RS24440	84382..85707	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
IB283_RS24445	85768..85974	+	207	WP_000134171.1	hypothetical protein	-
IB283_RS24450	86076..86486	+	411	WP_000985911.1	hypothetical protein	-
IB283_RS24455	86499..87314	+	816	WP_001572439.1	HNH endonuclease	-
IB283_RS24460	87568..87993	+	426	WP_001043843.1	hypothetical protein	-
IB283_RS24465	88447..88683	+	237	Protein_85	hypothetical protein	-
IB283_RS24470	88738..89037	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / qnrS1 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 / floR	-	1..157028	157028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T172205 WP_000088046.1 NZ_CP061187:84382-85707 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T172205 NZ_CP079645:c4934431-4934147 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT172205 WP_024136327.1 NZ_CP061187:84084-84377 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT172205 NZ_CP079645:c4934663-4934421 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGTTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1