172204

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6855..7348 Replicon plasmid pLD67-1-MCR1
Accession NZ_CP061186
Organism Escherichia coli strain LD67-1

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag IB283_RS23680 Protein ID WP_001230709.1
Coordinates 6855..7109 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag IB283_RS23685 Protein ID WP_000151456.1
Coordinates 7106..7348 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB283_RS23645 2433..2594 + 162 WP_001434039.1 type I toxin-antitoxin system Hok family toxin -
IB283_RS23650 2743..3339 + 597 WP_000125165.1 hypothetical protein -
IB283_RS23655 3357..3641 - 285 WP_001162371.1 hypothetical protein -
IB283_RS23660 3715..4380 - 666 WP_015387337.1 P-loop NTPase -
IB283_RS23665 4967..5296 + 330 WP_000855119.1 hypothetical protein -
IB283_RS23670 5697..6212 + 516 WP_001025393.1 J domain-containing protein -
IB283_RS23675 6316..6858 + 543 WP_001083904.1 hypothetical protein -
IB283_RS23680 6855..7109 + 255 WP_001230709.1 hypothetical protein Toxin
IB283_RS23685 7106..7348 + 243 WP_000151456.1 hypothetical protein Antitoxin
IB283_RS23690 7485..7817 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
IB283_RS23695 7864..8739 - 876 WP_063859951.1 class A extended-spectrum beta-lactamase CTX-M-132 -
IB283_RS23700 8992..10254 - 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
IB283_RS23705 10639..10888 + 250 Protein_13 hypothetical protein -
IB283_RS23710 11004..11291 + 288 WP_000673430.1 hypothetical protein -
IB283_RS23715 11301..11717 + 417 WP_188322781.1 phage tail protein -
IB283_RS23720 11714..12025 + 312 WP_001310436.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-132 / mcr-1.1 - 1..66568 66568
- flank IS/Tn blaCTX-M-132 - 7864..10254 2390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T172204 WP_001230709.1 NZ_CP061186:6855-7109 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T172204 NZ_CP079645:c4802440-4801907 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGATTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT172204 WP_000151456.1 NZ_CP061186:7106-7348 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT172204 NZ_CP079645:c4802717-4802451 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References