Detailed information of TA system TA172195

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3997084..3997916
• Replicon: chromosome
• Accession: NZ_CP061185
• Organism: Escherichia coli strain LD67-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: IB283_RS18805
• Protein ID: WP_000854753.1
• Coordinates: 3997542..3997916 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: IB283_RS18800
• Protein ID: WP_001278232.1
• Coordinates: 3997084..3997452 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB283_RS18765	3992927..3993607	+	681	WP_001593697.1	WYL domain-containing protein	-
IB283_RS18770	3993755..3994432	+	678	WP_001097305.1	hypothetical protein	-
IB283_RS18775	3994438..3994671	+	234	WP_001278287.1	DUF905 family protein	-
IB283_RS18780	3994770..3995588	+	819	WP_001234631.1	DUF945 domain-containing protein	-
IB283_RS18785	3995670..3996149	+	480	WP_000860065.1	antirestriction protein	-
IB283_RS18790	3996161..3996637	+	477	WP_001186710.1	RadC family protein	-
IB283_RS18795	3996700..3996921	+	222	WP_000692303.1	DUF987 domain-containing protein	-
IB283_RS18800	3997084..3997452	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
IB283_RS18805	3997542..3997916	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
IB283_RS18810	3997913..3998401	+	489	WP_000777541.1	hypothetical protein	-
IB283_RS18815	3998413..3998610	+	198	WP_000839281.1	DUF957 domain-containing protein	-
IB283_RS18820	3998707..3999276	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
IB283_RS18825	3999618..3999788	+	171	Protein_3674	IS110 family transposase	-
IB283_RS18830	4000599..4001582	+	984	WP_001361242.1	KpsF/GutQ family sugar-phosphate isomerase	-
IB283_RS18835	4001654..4002802	+	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3999684..3999788	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T172195 WP_000854753.1 NZ_CP061185:3997542-3997916 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T172195 NZ_CP079644:c46063-45647 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT172195 WP_001278232.1 NZ_CP061185:3997084-3997452 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT172195 NZ_CP079644:c46290-46060 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72