Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3819963..3820617 Replicon chromosome
Accession NZ_CP061185
Organism Escherichia coli strain LD67-1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag IB283_RS17940 Protein ID WP_000244781.1
Coordinates 3819963..3820370 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IB283_RS17945 Protein ID WP_000354046.1
Coordinates 3820351..3820617 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB283_RS17920 3815920..3817653 - 1734 WP_077516661.1 single-stranded-DNA-specific exonuclease RecJ -
IB283_RS17925 3817659..3818369 - 711 WP_077516663.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IB283_RS17930 3818394..3819290 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
IB283_RS17935 3819402..3819923 + 522 WP_032303736.1 flavodoxin FldB -
IB283_RS17940 3819963..3820370 - 408 WP_000244781.1 protein YgfX Toxin
IB283_RS17945 3820351..3820617 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IB283_RS17950 3820860..3821840 + 981 WP_077516665.1 tRNA-modifying protein YgfZ -
IB283_RS17955 3821917..3822576 - 660 WP_000250269.1 hemolysin III family protein -
IB283_RS17960 3822740..3823051 - 312 WP_077516667.1 N(4)-acetylcytidine aminohydrolase -
IB283_RS17965 3823096..3824529 + 1434 WP_136698981.1 6-phospho-beta-glucosidase BglA -
IB283_RS17970 3824577..3825311 - 735 WP_077516695.1 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T172194 WP_000244781.1 NZ_CP061185:c3820370-3819963 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T172194 NZ_CP079642:c5362451-5362068 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172194 WP_000354046.1 NZ_CP061185:c3820617-3820351 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172194 NZ_CP079642:c5362693-5362451 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References