Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2246176..2246814 Replicon chromosome
Accession NZ_CP061185
Organism Escherichia coli strain LD67-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IB283_RS10460 Protein ID WP_000813794.1
Coordinates 2246176..2246352 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IB283_RS10465 Protein ID WP_001270286.1
Coordinates 2246398..2246814 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB283_RS10440 2241798..2243009 - 1212 WP_071788120.1 BenE family transporter YdcO -
IB283_RS10445 2243062..2243598 + 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
IB283_RS10450 2243671..2245632 + 1962 WP_023909195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IB283_RS10455 2245724..2245954 - 231 WP_000491567.1 DUF2554 family protein -
IB283_RS10460 2246176..2246352 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IB283_RS10465 2246398..2246814 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IB283_RS10470 2246893..2248299 + 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
IB283_RS10475 2248544..2249689 + 1146 WP_000047466.1 ABC transporter substrate-binding protein -
IB283_RS10480 2249707..2250720 + 1014 WP_022645666.1 ABC transporter ATP-binding protein -
IB283_RS10485 2250721..2251662 + 942 WP_088129997.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T172183 WP_000813794.1 NZ_CP061185:2246176-2246352 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T172183 NZ_CP079638:c46063-45647 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT172183 WP_001270286.1 NZ_CP061185:2246398-2246814 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT172183 NZ_CP079638:c46290-46060 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References