Detailed information of TA system TA172176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1843374..1844158
• Replicon: chromosome
• Accession: NZ_CP061185
• Organism: Escherichia coli strain LD67-1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: IB283_RS08495
• Protein ID: WP_000613626.1
• Coordinates: 1843374..1843868 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: IB283_RS08500
• Protein ID: WP_139352217.1
• Coordinates: 1843865..1844158 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB283_RS08475	1838567..1839664	+	1098	WP_000589300.1	flagellar basal body P-ring protein FlgI	-
IB283_RS08480	1839664..1840605	+	942	WP_012311956.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
IB283_RS08485	1840671..1842314	+	1644	WP_077516765.1	flagellar hook-associated protein FlgK	-
IB283_RS08490	1842326..1843279	+	954	WP_188322772.1	flagellar hook-associated protein FlgL	-
IB283_RS08495	1843374..1843868	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
IB283_RS08500	1843865..1844158	-	294	WP_139352217.1	DUF1778 domain-containing protein	Antitoxin
IB283_RS08505	1844292..1847477	-	3186	WP_077516769.1	ribonuclease E	-
IB283_RS08510	1848050..1849009	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T172176 WP_000613626.1 NZ_CP061185:c1843868-1843374 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T172176 NZ_CP079636:2014685-2014787 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11139.76 Da
• Isoelectric Point: 10.5423

>AT172176 WP_139352217.1 NZ_CP061185:c1844158-1843865 [Escherichia coli]
MPAANNIPARREFLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT172176 NZ_CP079636:c2014794-2014649 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: V0T0H9