Detailed information of TA system TA172175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1588841..1589546
• Replicon: chromosome
• Accession: NZ_CP061185
• Organism: Escherichia coli strain LD67-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: IB283_RS07300
• Protein ID: WP_000539521.1
• Coordinates: 1589160..1589546 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IB283_RS07295
• Protein ID: WP_001280945.1
• Coordinates: 1588841..1589170 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB283_RS07280	1583999..1584910	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
IB283_RS07285	1585087..1587435	+	2349	WP_022645413.1	EAL domain-containing protein	-
IB283_RS07290	1587443..1588771	+	1329	WP_022645414.1	GGDEF domain-containing protein	-
IB283_RS07295	1588841..1589170	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
IB283_RS07300	1589160..1589546	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IB283_RS07305	1589772..1591097	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
IB283_RS07310	1591310..1591693	+	384	WP_000497137.1	biofilm formation regulator BssR	-
IB283_RS07315	1591804..1592919	+	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
IB283_RS07320	1592916..1593542	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T172175 WP_000539521.1 NZ_CP061185:c1589546-1589160 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T172175 NZ_CP079636:791206-791616 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATAGCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT172175 WP_001280945.1 NZ_CP061185:c1589170-1588841 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT172175 NZ_CP079636:790959-791225 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6