Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 707740..708290 Replicon chromosome
Accession NZ_CP061185
Organism Escherichia coli strain LD67-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag IB283_RS03180 Protein ID WP_001160966.1
Coordinates 707976..708290 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag IB283_RS03175 Protein ID WP_000125568.1
Coordinates 707740..707973 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB283_RS03150 702814..703101 + 288 WP_000203741.1 ferredoxin-like protein FixX -
IB283_RS03155 703159..704490 + 1332 WP_001183201.1 MFS transporter -
IB283_RS03160 704598..705128 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
IB283_RS03165 705121..706983 + 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
IB283_RS03170 707175..707654 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
IB283_RS03175 707740..707973 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
IB283_RS03180 707976..708290 + 315 WP_001160966.1 CcdB family protein Toxin
IB283_RS03185 708287..709135 - 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
IB283_RS03190 709142..709519 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
IB283_RS03195 709522..710343 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
IB283_RS03200 710340..711329 - 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
IB283_RS03205 711329..712615 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T172171 WP_001160966.1 NZ_CP061185:707976-708290 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T172171 NZ_CP079634:3490776-3491186 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATAGCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT172171 WP_000125568.1 NZ_CP061185:707740-707973 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT172171 NZ_CP079634:3490529-3490795 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References