Detailed information of TA system TA172168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-couple_hipB
• Location: 1631..2286
• Replicon: plasmid p546-4
• Accession: NZ_CP061181
• Organism: Roseomonas sp. 546

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IAI59_RS22090
• Protein ID: WP_207419900.1
• Coordinates: 2038..2286 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IAI59_RS22085
• Protein ID: WP_207419901.1
• Coordinates: 1631..2038 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI59_RS22070	1..314	+	314	Protein_0	IS21 family transposase	-
IAI59_RS22075	247..975	+	729	WP_207419048.1	IS21-like element helper ATPase IstB	-
IAI59_RS22080	1050..1634	+	585	Protein_2	DNA adenine methylase	-
IAI59_RS22085	1631..2038	-	408	WP_207419901.1	helix-turn-helix domain-containing protein	Antitoxin
IAI59_RS22090	2038..2286	-	249	WP_207419900.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAI59_RS22095	2351..2596	-	246	WP_207419899.1	hypothetical protein	-
IAI59_RS22100	2615..2866	-	252	WP_207419898.1	hypothetical protein	-
IAI59_RS22105	2863..3153	-	291	WP_207419897.1	hypothetical protein	-
IAI59_RS22110	3150..3509	-	360	WP_207419896.1	hypothetical protein	-
IAI59_RS22115	3740..5050	+	1311	WP_207419895.1	hypothetical protein	-
IAI59_RS22120	5983..6543	-	561	WP_207419894.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64273	64273
	-	flank	IS/Tn	-	-	247..975	728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (73-127)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9156.60 Da
• Isoelectric Point: 11.3231

>T172168 WP_207419900.1 NZ_CP061181:c2286-2038 [Roseomonas sp. 546]
MILTRPALKDLGNVPKRERAQLTARLEAIAAAPTGSHGNVIAMKGEPAGRLRVRQGDWRAIFTIDGDDVVVIRIRHRREV
YD

Nucleotide

• Length: 249 bp

>T172168 NZ_CP079634:c2139720-2139436 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 13951.79 Da
• Isoelectric Point: 4.5175

>AT172168 WP_207419901.1 NZ_CP061181:c2038-1631 [Roseomonas sp. 546]
MAHIGPLAETADTVTLRRADFEALVAAAEDVADRASFAAFEARVARDGMAAVKADSLTLQEAVRILDEGESPVRVWREKR
GLTGRALAAAAGVSPVYLSEIENGKKPGSAAAIVALAKALQVDAGDLLPPAATDD

Nucleotide

• Length: 408 bp

>AT172168 NZ_CP079634:c2139952-2139710 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGTTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA