Detailed information of TA system TA172165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1390304..1390912
• Replicon: chromosome
• Accession: NZ_CP061172
• Organism: Paenibacillus peoriae strain ZF390

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: IAQ67_RS06300
• Protein ID: WP_007429335.1
• Coordinates: 1390562..1390912 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IAQ67_RS06295
• Protein ID: WP_170970806.1
• Coordinates: 1390304..1390555 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAQ67_RS06275	1385588..1386598	-	1011	WP_190298832.1	ABC transporter substrate-binding protein	-
IAQ67_RS06280	1386832..1387137	+	306	Protein_1202	LysE family transporter	-
IAQ67_RS06285	1387249..1388526	+	1278	WP_103048858.1	outer membrane lipoprotein carrier protein LolA	-
IAQ67_RS06290	1388784..1389980	+	1197	WP_061829579.1	alanine racemase	-
IAQ67_RS06295	1390304..1390555	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
IAQ67_RS06300	1390562..1390912	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IAQ67_RS06305	1391092..1393314	+	2223	WP_180984597.1	RNA-binding transcriptional accessory protein	-
IAQ67_RS06310	1393420..1393554	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
IAQ67_RS06315	1393699..1394223	+	525	WP_103048857.1	SprT family protein	-
IAQ67_RS06330	1394611..1395486	-	876	WP_190298833.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T172165 WP_007429335.1 NZ_CP061172:1390562-1390912 [Paenibacillus peoriae]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T172165 NZ_CP079634:c1274690-1274373 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT172165 WP_170970806.1 NZ_CP061172:1390304-1390555 [Paenibacillus peoriae]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT172165 NZ_CP079634:c1274380-1274093 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: H6CFY1