Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509114..509751 Replicon chromosome
Accession NZ_CP061168
Organism Bacillus amyloliquefaciens strain T-5

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IAU66_RS02435 Protein ID WP_003156187.1
Coordinates 509401..509751 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag IAU66_RS02430 Protein ID WP_003156188.1
Coordinates 509114..509395 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAU66_RS02410 505479..506078 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
IAU66_RS02415 506171..506536 + 366 WP_014304402.1 holo-ACP synthase -
IAU66_RS02420 506701..507708 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
IAU66_RS02425 507825..508994 + 1170 WP_003156189.1 alanine racemase -
IAU66_RS02430 509114..509395 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IAU66_RS02435 509401..509751 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IAU66_RS02440 509869..510690 + 822 WP_014304404.1 STAS domain-containing protein -
IAU66_RS02445 510695..511060 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
IAU66_RS02450 511063..511464 + 402 WP_003156178.1 anti-sigma regulatory factor -
IAU66_RS02455 511476..512483 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
IAU66_RS02460 512547..512876 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
IAU66_RS02465 512873..513355 + 483 WP_003156173.1 anti-sigma B factor RsbW -
IAU66_RS02470 513321..514109 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
IAU66_RS02475 514109..514711 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T172154 WP_003156187.1 NZ_CP061168:509401-509751 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T172154 NZ_CP079629:2138967-2139069 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT172154 WP_003156188.1 NZ_CP061168:509114-509395 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT172154 NZ_CP079629:c2139076-2138931 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References