Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 85381..86024 Replicon plasmid pEC187_1
Accession NZ_CP061109
Organism Escherichia coli strain 1EC187

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag IBZ91_RS23660 Protein ID WP_001034046.1
Coordinates 85608..86024 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag IBZ91_RS23655 Protein ID WP_001261278.1
Coordinates 85381..85611 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBZ91_RS23625 80892..81197 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
IBZ91_RS23630 81199..81417 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
IBZ91_RS23635 81984..82496 + 513 WP_000151784.1 hypothetical protein -
IBZ91_RS23640 82530..83663 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
IBZ91_RS23645 83830..84603 - 774 WP_000905949.1 hypothetical protein -
IBZ91_RS23650 84616..85116 - 501 WP_000528931.1 hypothetical protein -
IBZ91_RS23655 85381..85611 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IBZ91_RS23660 85608..86024 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
IBZ91_RS23665 86069..89863 - 3795 WP_199212461.1 pcar -
IBZ91_RS23670 90244..90474 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IBZ91_RS23675 90471..90887 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / catA1 / dfrA14 / mph(A) / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD iutA / iucD / iucC / iucB / iucA 1..112598 112598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T172141 WP_001034046.1 NZ_CP061109:85608-86024 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T172141 NZ_CP079624:2153507-2153609 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT172141 WP_001261278.1 NZ_CP061109:85381-85611 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT172141 NZ_CP079624:c2153616-2153471 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References