Detailed information of TA system TA172140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 80892..81417
• Replicon: plasmid pEC187_1
• Accession: NZ_CP061109
• Organism: Escherichia coli strain 1EC187

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: IBZ91_RS23625
• Protein ID: WP_001159868.1
• Coordinates: 80892..81197 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: IBZ91_RS23630
• Protein ID: WP_000813634.1
• Coordinates: 81199..81417 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBZ91_RS23610	76802..77968	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
IBZ91_RS23615	78556..79311	-	756	WP_000852146.1	replication initiation protein RepE	-
IBZ91_RS23620	80085..80891	-	807	WP_000016982.1	site-specific integrase	-
IBZ91_RS23625	80892..81197	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
IBZ91_RS23630	81199..81417	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IBZ91_RS23635	81984..82496	+	513	WP_000151784.1	hypothetical protein	-
IBZ91_RS23640	82530..83663	-	1134	WP_000545987.1	DUF3800 domain-containing protein	-
IBZ91_RS23645	83830..84603	-	774	WP_000905949.1	hypothetical protein	-
IBZ91_RS23650	84616..85116	-	501	WP_000528931.1	hypothetical protein	-
IBZ91_RS23655	85381..85611	+	231	WP_001261278.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
IBZ91_RS23660	85608..86024	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / catA1 / dfrA14 / mph(A) / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA	1..112598	112598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T172140 WP_001159868.1 NZ_CP061109:c81197-80892 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T172140 NZ_CP079624:849130-849540 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGAAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT172140 WP_000813634.1 NZ_CP061109:c81417-81199 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT172140 NZ_CP079624:848883-849149 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2