Detailed information of TA system TA172136

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4327054..4327886
• Replicon: chromosome
• Accession: NZ_CP061108
• Organism: Escherichia coli strain 1EC187

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2R9V8K2
• Locus tag: IBZ91_RS20690
• Protein ID: WP_001468432.1
• Coordinates: 4327512..4327886 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: IBZ91_RS20685
• Protein ID: WP_001285575.1
• Coordinates: 4327054..4327422 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBZ91_RS20650	4322646..4322963	-	318	Protein_4037	transposase	-
IBZ91_RS20655	4323054..4323440	-	387	WP_000631802.1	toxin-immunity protein system imunity protein CdiI	-
IBZ91_RS20660	4323437..4325392	-	1956	Protein_4039	VENN motif pre-toxin domain-containing protein	-
IBZ91_RS20665	4325387..4325599	+	213	Protein_4040	DUF932 domain-containing protein	-
IBZ91_RS20670	4325690..4326175	+	486	WP_000213697.1	antirestriction protein	-
IBZ91_RS20675	4326190..4326666	+	477	WP_001298074.1	RadC family protein	-
IBZ91_RS20680	4326753..4326974	+	222	WP_000692350.1	DUF987 domain-containing protein	-
IBZ91_RS20685	4327054..4327422	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IBZ91_RS20690	4327512..4327886	+	375	WP_001468432.1	TA system toxin CbtA family protein	Toxin
IBZ91_RS20695	4327883..4328371	+	489	WP_000777545.1	hypothetical protein	-
IBZ91_RS20700	4328383..4328580	+	198	WP_000839281.1	DUF957 domain-containing protein	-
IBZ91_RS20705	4328677..4329246	+	570	WP_001290252.1	DUF4942 domain-containing protein	-
IBZ91_RS20710	4329995..4331533	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papX / papF / papE / papK / papJ / papD / papC / papH / papA / papB / papI / cnf1 / hlyD / hlyB / hlyA / hlyC	4259013..4339346	80333
	-	flank	IS/Tn	-	-	4322646..4323023	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14102.04 Da
• Isoelectric Point: 7.3526

>T172136 WP_001468432.1 NZ_CP061108:4327512-4327886 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T172136 NZ_CP079617:c4936141-4935857 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT172136 WP_001285575.1 NZ_CP061108:4327054-4327422 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT172136 NZ_CP079617:c4936373-4936131 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGTTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A2R9V8K2

Antitoxin

• AlphaFold DB: A0A454ABF5