Detailed information of TA system TA172124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1347384..1348009
• Replicon: chromosome
• Accession: NZ_CP061108
• Organism: Escherichia coli strain 1EC187

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IBZ91_RS06485
• Protein ID: WP_000911330.1
• Coordinates: 1347611..1348009 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IBZ91_RS06480
• Protein ID: WP_000450524.1
• Coordinates: 1347384..1347611 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBZ91_RS06455	1343187..1343657	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
IBZ91_RS06460	1343657..1344229	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IBZ91_RS06465	1344375..1345253	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IBZ91_RS06470	1345270..1346304	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
IBZ91_RS06475	1346517..1347230	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IBZ91_RS06480	1347384..1347611	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IBZ91_RS06485	1347611..1348009	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IBZ91_RS06490	1348156..1349019	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
IBZ91_RS06495	1349034..1351049	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
IBZ91_RS06500	1351123..1351821	+	699	WP_000679823.1	esterase	-
IBZ91_RS06505	1351931..1352131	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T172124 WP_000911330.1 NZ_CP061108:1347611-1348009 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T172124 NZ_CP079606:3849684-3850094 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATAGCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT172124 WP_000450524.1 NZ_CP061108:1347384-1347611 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT172124 NZ_CP079606:3849437-3849703 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33