Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 329121..329921 Replicon chromosome
Accession NZ_CP061108
Organism Escherichia coli strain 1EC187

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag IBZ91_RS01490 Protein ID WP_000342449.1
Coordinates 329394..329921 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag IBZ91_RS01485 Protein ID WP_001277108.1
Coordinates 329121..329387 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBZ91_RS01465 324778..325446 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
IBZ91_RS01470 325439..326497 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
IBZ91_RS01475 326742..327596 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
IBZ91_RS01480 327868..328971 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
IBZ91_RS01485 329121..329387 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
IBZ91_RS01490 329394..329921 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
IBZ91_RS01495 329918..330301 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
IBZ91_RS01500 330725..331834 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
IBZ91_RS01505 331882..332808 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IBZ91_RS01510 332805..334082 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
IBZ91_RS01515 334079..334846 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T172118 WP_000342449.1 NZ_CP061108:329394-329921 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T172118 NZ_CP079606:c1625963-1625646 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT172118 WP_001277108.1 NZ_CP061108:329121-329387 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT172118 NZ_CP079606:c1625653-1625366 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References