Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 37578..38179 Replicon plasmid pEC213_2
Accession NZ_CP061103
Organism Escherichia coli strain 1EC213

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag IB009_RS23600 Protein ID WP_001216041.1
Coordinates 37578..37958 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IB009_RS23605 Protein ID WP_001190712.1
Coordinates 37958..38179 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB009_RS23570 33600..34628 + 1029 WP_088548881.1 tyrosine-type recombinase/integrase -
IB009_RS23575 34738..35046 + 309 WP_000349257.1 hypothetical protein -
IB009_RS23580 35043..35519 + 477 WP_000861173.1 hypothetical protein -
IB009_RS23585 35516..36010 + 495 WP_000640903.1 dUTP diphosphatase -
IB009_RS23590 36025..36687 + 663 WP_000255077.1 DUF2829 domain-containing protein -
IB009_RS23595 36694..37494 + 801 WP_000432092.1 hypothetical protein -
IB009_RS23600 37578..37958 - 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IB009_RS23605 37958..38179 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IB009_RS23610 38252..38641 - 390 WP_000506731.1 hypothetical protein -
IB009_RS23615 38765..39016 - 252 WP_001283837.1 DNA polymerase III subunit theta -
IB009_RS23620 39190..39399 + 210 WP_199206747.1 hypothetical protein -
IB009_RS23625 39384..39668 - 285 WP_001142396.1 hypothetical protein -
IB009_RS23630 39652..40425 - 774 Protein_41 hypothetical protein -
IB009_RS23635 40710..40919 - 210 WP_000951710.1 hypothetical protein -
IB009_RS23640 40921..41109 - 189 WP_000797282.1 hypothetical protein -
IB009_RS23645 41106..41282 - 177 WP_001261535.1 hypothetical protein -
IB009_RS23650 41282..41995 - 714 WP_069067276.1 ead/Ea22-like family protein -
IB009_RS23655 41992..42678 - 687 WP_199206743.1 ead/Ea22-like family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92656 92656


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T172116 WP_001216041.1 NZ_CP061103:c37958-37578 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T172116 NZ_CP079600:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT172116 WP_001190712.1 NZ_CP061103:c38179-37958 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT172116 NZ_CP079600:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References