Detailed information of TA system TA172108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 47579..48126
• Replicon: plasmid pEC213_1-OXA-181
• Accession: NZ_CP061102
• Organism: Escherichia coli strain 1EC213

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: IB009_RS23130
• Protein ID: WP_000969980.1
• Coordinates: 47579..47860 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6G2GFN7
• Locus tag: IB009_RS23135
• Protein ID: WP_000079949.1
• Coordinates: 47857..48126 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB009_RS23105	42700..43257	-	558	WP_001235713.1	recombinase family protein	-
IB009_RS23110	43421..43990	+	570	Protein_41	DUF4158 domain-containing protein	-
IB009_RS23115	44055..44759	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IB009_RS23120	44807..47254	+	2448	Protein_43	Tn3-like element Tn3 family transposase	-
IB009_RS23125	47288..47533	-	246	Protein_44	3'-5' exonuclease	-
IB009_RS23130	47579..47860	-	282	WP_000969980.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IB009_RS23135	47857..48126	-	270	WP_000079949.1	hypothetical protein	Antitoxin
IB009_RS23140	49041..49898	-	858	WP_000774301.1	incFII family plasmid replication initiator RepA	-
IB009_RS23145	49891..50373	-	483	WP_001443034.1	hypothetical protein	-
IB009_RS23150	50366..50440	-	75	WP_001442103.1	RepA leader peptide Tap	-
IB009_RS23155	50674..50931	-	258	WP_000083834.1	replication regulatory protein RepA	-
IB009_RS23160	51215..51421	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	-
-	51408..51469	+	62	NuclAT_1	-	-
-	51408..51469	+	62	NuclAT_1	-	-
-	51408..51469	+	62	NuclAT_1	-	-
-	51408..51469	+	62	NuclAT_1	-	-
IB009_RS23165	51765..52076	+	312	WP_000802277.1	hypothetical protein	-
IB009_RS23170	52257..52343	-	87	Protein_53	endonuclease	-
IB009_RS23175	52541..52729	-	189	WP_001490801.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaOXA-181 / qnrS1 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-35	-	1..95832	95832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10829.52 Da
• Isoelectric Point: 8.5042

>T172108 WP_000969980.1 NZ_CP061102:c47860-47579 [Escherichia coli]
MMEIFWTMLASLDRKRIRENIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T172108 NZ_CP079599:c2805890-2805480 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGAAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10205.53 Da
• Isoelectric Point: 7.3210

>AT172108 WP_000079949.1 NZ_CP061102:c48126-47857 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVTAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT172108 NZ_CP079599:c2806137-2805871 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G2GFN7