Detailed information of TA system TA172105

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4060447..4061279
• Replicon: chromosome
• Accession: NZ_CP061101
• Organism: Escherichia coli strain 1EC213

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: IB009_RS19725
• Protein ID: WP_000854765.1
• Coordinates: 4060447..4060821 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: IB009_RS19730
• Protein ID: WP_001295723.1
• Coordinates: 4060911..4061279 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB009_RS19690	4055624..4055794	-	171	Protein_3848	fimbrial protein	-
IB009_RS19695	4055849..4056546	+	698	Protein_3849	IS1 family transposase	-
IB009_RS19700	4056922..4057080	-	159	WP_001467148.1	hypothetical protein	-
IB009_RS19705	4057180..4057356	-	177	WP_000839290.1	DUF957 domain-containing protein	-
IB009_RS19710	4057480..4058226	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
IB009_RS19715	4058241..4059782	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
IB009_RS19720	4059923..4060450	-	528	WP_123002132.1	hypothetical protein	-
IB009_RS19725	4060447..4060821	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
IB009_RS19730	4060911..4061279	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IB009_RS19735	4061442..4061663	-	222	WP_000692311.1	DUF987 domain-containing protein	-
IB009_RS19740	4061726..4062202	-	477	WP_001186774.1	RadC family protein	-
IB009_RS19745	4062218..4062703	-	486	WP_000849588.1	antirestriction protein	-
IB009_RS19750	4062758..4063576	-	819	WP_001234620.1	DUF945 domain-containing protein	-
IB009_RS19755	4063676..4063909	-	234	WP_001119729.1	DUF905 family protein	-
IB009_RS19760	4063988..4064443	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T172105 WP_000854765.1 NZ_CP061101:c4060821-4060447 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T172105 NZ_CP079236:1272478-1272732 [Bifidobacterium pseudocatenulatum]
ATAATGCTGCGTTGGACTGATGACGCGTGGAACGATTACCTGTATTGGCAGGTTCAGGATAAGAAAACCCTCAAGCGGGT
CAACGCGCTGATACGTGATGCGATGCGTTCTCCATTCGCGGGTCTCGGTAAGCCAGAGCCGCTCAAATGGGAACTGCAGG
GTGCGTGGAGCCGCCGCATCGATTCCGCAAACCGTCTGGTATACATGATTGAGGACGGCGACTTGTGCATTCTGTCCGTC
CGTGACCACTATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT172105 WP_001295723.1 NZ_CP061101:c4061279-4060911 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT172105 NZ_CP079236:1272218-1272478 [Bifidobacterium pseudocatenulatum]
ATGGTCACGGCGGTCGCGTACACCAATTTCCGCAAGGATCTGAAGTCATATCTACGTCGCGTGAACGATGACGCGGATAT
GCTTCTGATTACCAACAAGGATCCAGGGGACAACGTGGTGGTGATGAGCGCCGATGATTACGATTCGCTTATGGAGACAT
TGCGCGTTTACCAGAATCCCGGATTGTTCGATAAAGTCCGCCGTGGCATGGCACAGGTACGCTCAGGTTCCACCGATTTA
CATGATCTGGTCGGAGAGTGA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52