Detailed information of TA system TA172101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3546530..3547367
• Replicon: chromosome
• Accession: NZ_CP061101
• Organism: Escherichia coli strain 1EC213

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: IB009_RS17315
• Protein ID: WP_000227784.1
• Coordinates: 3546825..3547367 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: IB009_RS17310
• Protein ID: WP_001297137.1
• Coordinates: 3546530..3546841 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB009_RS17285	3541550..3542497	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
IB009_RS17290	3542519..3544510	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
IB009_RS17295	3544500..3545114	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
IB009_RS17300	3545114..3545443	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
IB009_RS17305	3545455..3546345	+	891	WP_000971336.1	heme o synthase	-
IB009_RS17310	3546530..3546841	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
IB009_RS17315	3546825..3547367	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
IB009_RS17320	3547423..3548358	-	936	WP_001297127.1	sel1 repeat family protein	-
IB009_RS17325	3548766..3550130	+	1365	WP_001000978.1	MFS transporter	-
IB009_RS17330	3550258..3550749	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
IB009_RS17335	3550917..3551828	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T172101 WP_000227784.1 NZ_CP061101:3546825-3547367 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T172101 NZ_CP079225:c2783646-2783077 [Rhodococcus sp. LW-XY12]
ATGAGGCGTCTGGATCTGATCGTCGGTCCCAACGGTTCCGGCAAGTCGACGTTCGTCGAGTTGTCCCTCGCTCCCCTGCT
GCCGCGCAGCGTGTTCGTCAATGCGGATGTGATCGCGCGGCAGCGCTGGCCCGACCGGGCGGAGGAGATGTCGTACGAGG
CGGCGCAACTCGCCGCGGCGGCGCGGGACCGGTTGCTGCGGCAGGGCCGGTCGTTCATTGCGGAGACGGTGTTCTCGCAT
CCGTCGAAGCTGGAGTTGATCGACCGCGCGCACGAGGTGGGGTTCACGGTGGTGCTGCACGTGATGCTGGTGCCCGAGGC
GATGTCGGTGCTGCGTGTGCAGCACCGGGTGGCCTCCGGTGGGCATGCGGTGCCCGAGGACAAGATCCGGGCGCGTTATC
GGCGGTTGTGGCCGCTGGTGGCCGAAGCGATCGGCCGCGCCGATCAGGCGTCGGTGTACGACACGGCGTCCGGGACCCCG
GTGGTGGTGGCGCGGTTCGCCGGCGGGGTGCCGGTGGGTGTGGTGCGCTGGCCGGGATGGGCGCCGGCGGCGCTCACCGG
GCTCGGCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT172101 WP_001297137.1 NZ_CP061101:3546530-3546841 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT172101 NZ_CP079225:c2784029-2783643 [Rhodococcus sp. LW-XY12]
ATGACGTCATCCACCGACAAGGTCACCCGCTTCGACGCCGAACTCGTCGACAGCGCCATCGCCGAGGGCGGCCGCCAGAA
CCGCACCGGACGCCAGCAACTCGAATACTGGGCCCGCATCGGCCGCGCGATGACCGCCCACGAGACCGCCTCCCTGCACC
GGGTGCACGAGGCGCTGGCCGGGACACGGGAGCTGAGCGAGCTCACCGCCGCCGAGGGACGGCTGTTCGACGCAGAGATC
GATGCGCGCCTGGCGGGCGGGCTGGCCCGCACCGACTACGCCGAGGTTCTCGCCGCGCGTGGGGTCACCACGGTGGTGCT
CGACGACGAAGGCCGGCTGGTCGAGCAGCGTCCCGACGGTAGTCGCCGGGTGCTCGACGACGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3