Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3512453..3513071 Replicon chromosome
Accession NZ_CP061101
Organism Escherichia coli strain 1EC213

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IB009_RS17145 Protein ID WP_001291435.1
Coordinates 3512853..3513071 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IB009_RS17140 Protein ID WP_000344800.1
Coordinates 3512453..3512827 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB009_RS17130 3507542..3508735 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IB009_RS17135 3508758..3511907 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IB009_RS17140 3512453..3512827 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IB009_RS17145 3512853..3513071 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IB009_RS17150 3513242..3513793 + 552 WP_000102564.1 maltose O-acetyltransferase -
IB009_RS17155 3513909..3514379 + 471 WP_000136192.1 YlaC family protein -
IB009_RS17160 3514543..3516093 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IB009_RS17165 3516135..3516488 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IB009_RS17175 3516867..3517178 + 312 WP_000409911.1 MGMT family protein -
IB009_RS17180 3517209..3517781 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T172100 WP_001291435.1 NZ_CP061101:3512853-3513071 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T172100 NZ_CP079225:652113-652391 [Rhodococcus sp. LW-XY12]
ATGAGCACTCCGGACCACCCGTATCGGCTGGTGATGGCGAGGTCGGCTGCACGCGCCATGGCGAGGTCGTTGCCGGAGAA
GGTAGCAACTGCGGTATACGAGTTCGTCACAGGACCCCTTCTCGAGAATCCGAAGCGGGTGGGGAAGCCGTTGAATCCGC
CTCTTGCGCCGGCGTACAGCGCCCGGCGAGGTGAATATCGCGTCCTGTATCTGATCGATGATGCGAACCGCACGGTCGAG
GTCACCGCGATTTCGCACCGTGCCGATGCGTATCGATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT172100 WP_000344800.1 NZ_CP061101:3512453-3512827 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT172100 NZ_CP079225:651832-652116 [Rhodococcus sp. LW-XY12]
ATGACGACTCTTCCTGTTGCCGACGCGCGGGCGCAATTGTCACGACTGGTCGATGAGGCCGAGCGCACACACGAGCGATT
CGAGATCACCCGCAACGGGCACCGCGCCGCGGTGCTTCTCGGTGCCGACGATTACGACGCGATGCAGGAGACGATCGCGG
TGTTGTCGGATACGGATCTGCTGCGGGCGCACGTCGAGGGCCGAACGGAGGTCGAGTCGGGAGACGTCGTCGATGTACAG
GAATTGACGCAGCTGATGCGTGATGCTCGGTCGTCCGAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References