Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1356975..1357600 Replicon chromosome
Accession NZ_CP061101
Organism Escherichia coli strain 1EC213

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag IB009_RS06620 Protein ID WP_000911329.1
Coordinates 1357202..1357600 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag IB009_RS06615 Protein ID WP_000450524.1
Coordinates 1356975..1357202 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB009_RS06590 1352776..1353246 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
IB009_RS06595 1353246..1353818 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
IB009_RS06600 1353964..1354842 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
IB009_RS06605 1354859..1355893 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
IB009_RS06610 1356106..1356819 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
IB009_RS06615 1356975..1357202 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
IB009_RS06620 1357202..1357600 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
IB009_RS06625 1357747..1358610 + 864 WP_001267507.1 neutral zinc metallopeptidase -
IB009_RS06630 1358625..1360640 + 2016 WP_074683898.1 tRNA cytosine(34) acetyltransferase TmcA -
IB009_RS06635 1360714..1361412 + 699 WP_000679812.1 esterase -
IB009_RS06640 1361532..1362512 - 981 WP_000399648.1 IS110-like element IS621 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1361532..1362512 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T172087 WP_000911329.1 NZ_CP061101:1357202-1357600 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T172087 NZ_CP079195:2877871-2878119 [Polaribacter sp. NJDZ03]
ATGGAAACAGTAAATTATACAGATTTTCGTTCCAATTTAAAACATTGGTTCGATAAGGTTTTTAATGATGTTAGTGATAT
CGTCATTAAAAGAAAAGGAGGAAAGGATTTAGTTCTAATTTCATTAGACGAATATAACTCTCTTAAGGAAACAAGTTATT
TGCTTACAGGGAAAAACAGAGATGTTTTATTAAATTCCATTAAAGAATTAGAAGCTGGCAAAGGATTTCAAAAAGAATTA
ATCGAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT172087 WP_000450524.1 NZ_CP061101:1356975-1357202 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT172087 NZ_CP079195:2878120-2878380 [Polaribacter sp. NJDZ03]
ATGAGATTAATTTGGTCAGCATCTTCTTGGAACGATTATGTCTATTGGCAAAAAGTTGATAAGAAAATTGTTAAAAGAAT
TAATGAACTTATTAAAAGTTGCGTTAGAACACCTTTTGAAGGGATTGGAAAACCAGAAGCTTTAAAAGGAGATTTACAAG
GGTATTGGTCTAGAAGAATAACATCTGAACATAGACTAGTATATAAATATGAGAATGAACAGCTATTAATTGCTGCTTGT
CGCTATCATTATGGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References