Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1026071..1026654 Replicon chromosome
Accession NZ_CP061101
Organism Escherichia coli strain 1EC213

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag IB009_RS04945 Protein ID WP_000254745.1
Coordinates 1026319..1026654 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3P5DUY8
Locus tag IB009_RS04940 Protein ID WP_074502498.1
Coordinates 1026071..1026319 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB009_RS04930 1022410..1023711 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
IB009_RS04935 1023759..1025993 + 2235 WP_000226815.1 GTP pyrophosphokinase -
IB009_RS04940 1026071..1026319 + 249 WP_074502498.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IB009_RS04945 1026319..1026654 + 336 WP_000254745.1 endoribonuclease MazF Toxin
IB009_RS04950 1026725..1027516 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IB009_RS04955 1027744..1029381 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IB009_RS04960 1029469..1030767 + 1299 WP_000036723.1 phosphopyruvate hydratase -
IB009_RS04965 1030823..1031185 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T172086 WP_000254745.1 NZ_CP061101:1026319-1026654 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T172086 NZ_CP079193:c1954075-1953746 [Streptococcus suis]
ATGGAATTTGAGTCATATTCAGTGATACTTGCTCCAGCAGTGGAGAAGGAATTGGCAGATATTTACGCCTATTTTTCGGA
ACAGTTCTCAGAAGAAATTGCCAAAAGACGAATTGGGATGATTGTCGAAGCATTGGAGAGTTTGCAAATTTTTCCGGAAC
GTGGCTTTAACGCCGATAACCGTTTTGGTAAGCAGATAGACCCGCCACATCTGACCCGAGGTTACGTTATTGGAAAAGAC
TACATTGCACTCTATCGTGTGGTCGAAAAAGAAGTGCGAGTTGGTCATCTCTTTGCGACTAAGAGTGATTATGTGAAGTT
GTTGAAGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9359.72 Da        Isoelectric Point: 4.4791

>AT172086 WP_074502498.1 NZ_CP061101:1026071-1026319 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGESKDKE
VW

Download         Length: 249 bp

>AT172086 NZ_CP079193:c1954271-1954065 [Streptococcus suis]
ATGAACCAGTTGGATTTGACTGCTGGTTTTAACCTCTTTTTACAAAACATTGCTGTAAAAAATGAGTTGCCCATTCTGAC
AGAAGAAGAGTTGGAAAGAGAAGAGTTGTTTTTACAACTTCAAAAGGAAATCCAGGAAAACCAACAGGCGATTGAGGCAG
GTCAAGGGATCAGCCTAGAAATCGTCAAAGAGAAGTATGGAATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A3P5DUY8

References