Detailed information of TA system TA172081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-PrlF
• Location: 16883..17438
• Replicon: plasmid p1318-5
• Accession: NZ_CP061096
• Organism: Roseomonas marmotae strain 1318

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IAI58_RS22095
• Protein ID: WP_208776333.1
• Coordinates: 17088..17438 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IAI58_RS22090
• Protein ID: WP_207451571.1
• Coordinates: 16883..17101 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI58_RS22060 (IAI58_22045)	12005..12949	+	945	WP_207451128.1	tripartite tricarboxylate transporter substrate binding protein	-
IAI58_RS22065 (IAI58_22050)	12961..13731	+	771	Protein_11	IS6 family transposase	-
IAI58_RS22070 (IAI58_22055)	13868..14116	-	249	WP_237182700.1	tripartite tricarboxylate transporter substrate-binding protein	-
IAI58_RS22075 (IAI58_22060)	14043..14447	-	405	WP_208776331.1	tripartite tricarboxylate transporter substrate-binding protein	-
IAI58_RS22080 (IAI58_22065)	14545..15456	-	912	WP_207451132.1	tripartite tricarboxylate transporter substrate-binding protein	-
IAI58_RS22085 (IAI58_22070)	15866..16786	+	921	WP_208776332.1	3'-5' exonuclease	-
IAI58_RS22090 (IAI58_22075)	16883..17101	+	219	WP_207451571.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
IAI58_RS22095 (IAI58_22080)	17088..17438	+	351	WP_208776333.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IAI58_RS22100 (IAI58_22085)	18523..21222	+	2700	WP_208776334.1	virulence-associated E family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..60587	60587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-110)

Antitoxin

• PrlF antitoxin (4-53)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12404.28 Da
• Isoelectric Point: 5.0807

>T172081 WP_208776333.1 NZ_CP061096:17088-17438 [Roseomonas marmotae]
MPGFEVWDLVKVPFPYTNRPVQQRRPALVIAAPEASGTPVLLWVLMVTSAENRGWPGDVTVSDLTEAGLPAPSVVRPAKI
ATIEAGDAERIGRLPEPDQRQVAQALRASLGGVLAQ

Nucleotide

• Length: 351 bp

>T172081 NZ_CP079188:53367-53672 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8032.09 Da
• Isoelectric Point: 6.2833

>AT172081 WP_207451571.1 NZ_CP061096:16883-17101 [Roseomonas marmotae]
MITSRLTSKAQTTIPQPVRAALRLREGDEIAYTIEQGRVVLTRVQPERAADDPFRTFAEWESPADAKAYAGL

Nucleotide

• Length: 219 bp

>AT172081 NZ_CP079188:53147-53365 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA