Detailed information of TA system TA172078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 326637..327225
• Replicon: chromosome
• Accession: NZ_CP061088
• Organism: Brucella sp. 6810

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IB024_RS01885
• Protein ID: WP_187960141.1
• Coordinates: 326935..327225 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7L7ML36
• Locus tag: IB024_RS01880
• Protein ID: WP_008505633.1
• Coordinates: 326637..326933 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB024_RS01830	322751..323119	-	369	WP_187960132.1	hypothetical protein	-
IB024_RS01835	323165..323449	-	285	WP_187960133.1	hypothetical protein	-
IB024_RS01840	323508..323810	-	303	WP_187960134.1	Arc family DNA-binding protein	-
IB024_RS01845	323909..324475	+	567	WP_187960135.1	phage antirepressor N-terminal domain-containing protein	-
IB024_RS01850	324475..324642	+	168	WP_187960136.1	Arc family DNA-binding protein	-
IB024_RS01855	324639..324863	+	225	WP_187960137.1	Arc family DNA-binding protein	-
IB024_RS01860	324850..325551	+	702	WP_210310607.1	Rha family transcriptional regulator	-
IB024_RS01865	325551..325790	+	240	WP_187960138.1	hypothetical protein	-
IB024_RS01870	325790..326077	+	288	WP_187960139.1	hypothetical protein	-
IB024_RS01875	326225..326548	-	324	WP_187960140.1	hypothetical protein	-
IB024_RS01880	326637..326933	-	297	WP_008505633.1	putative addiction module antidote protein	Antitoxin
IB024_RS01885	326935..327225	-	291	WP_187960141.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IB024_RS01890	327457..327741	-	285	WP_025200046.1	hypothetical protein	-
IB024_RS01895	327833..328630	+	798	WP_187960142.1	tail fiber protein	-
IB024_RS01900	328630..329853	+	1224	WP_187960143.1	hypothetical protein	-
IB024_RS01905	330024..330230	+	207	Protein_371	hypothetical protein	-
IB024_RS01910	330299..330982	+	684	WP_187960144.1	lysozyme	-
IB024_RS01915	330979..331227	+	249	WP_187960145.1	hypothetical protein	-
IB024_RS01920	331388..331630	+	243	WP_187960146.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	289846..343705	53859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10755.47 Da
• Isoelectric Point: 10.2779

>T172078 WP_187960141.1 NZ_CP061088:c327225-326935 [Brucella sp. 6810]
MIEVRQTTAFTKWLNELRDTNARLRIATRIRRMELGNPGDVKAVGEGVSEMRITYGQGYRVYFVTMGSTIVVLLCGGDKS
SQSRDIAVAKQMAKEI

Nucleotide

• Length: 291 bp

>T172078 NZ_CP079186:c5342760-5342377 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10440.88 Da
• Isoelectric Point: 5.1057

>AT172078 WP_008505633.1 NZ_CP061088:c326933-326637 [Brucella sp. 6810]
MALETTRFDILDHLKTPEERLAYLEAAFEDGDPSLIAHALGDVARSVGMTAVAKEAGITREALYRSLSENGDPKLSTLLG
VTKALGIHLTVGPDKHAA

Nucleotide

• Length: 297 bp

>AT172078 NZ_CP079186:c5343002-5342760 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7L7ML36