Detailed information of TA system TA172077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 227680..228268
• Replicon: chromosome
• Accession: NZ_CP061088
• Organism: Brucella sp. 6810

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7L7ML11
• Locus tag: IB024_RS01235
• Protein ID: WP_008505629.1
• Coordinates: 227978..228268 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7L7ML36
• Locus tag: IB024_RS01230
• Protein ID: WP_008505633.1
• Coordinates: 227680..227976 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB024_RS01205	222869..223997	+	1129	WP_011950188.1	peptide chain release factor 2	-
IB024_RS01210	224131..225630	+	1500	WP_187959937.1	IS21 family transposase	-
IB024_RS01215	225632..226366	+	735	WP_151653755.1	IS21-like element helper ATPase IstB	-
IB024_RS01220	226516..226698	+	183	WP_187960040.1	hypothetical protein	-
IB024_RS01225	226724..227620	-	897	WP_187960041.1	hypothetical protein	-
IB024_RS01230	227680..227976	-	297	WP_008505633.1	putative addiction module antidote protein	Antitoxin
IB024_RS01235	227978..228268	-	291	WP_008505629.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IB024_RS01240	228389..228655	-	267	WP_187960042.1	Arc family DNA-binding protein	-
IB024_RS01245	228767..229333	+	567	WP_187960043.1	phage P22, antirepressor protein	-
IB024_RS01250	229330..229554	+	225	WP_187960044.1	Arc family DNA-binding protein	-
IB024_RS01255	229556..230242	+	687	WP_210310604.1	Rha family transcriptional regulator	-
IB024_RS01260	230242..230481	+	240	WP_187960045.1	hypothetical protein	-
IB024_RS01265	230481..230849	+	369	WP_187960505.1	hypothetical protein	-
IB024_RS01270	231114..232199	+	1086	WP_187960046.1	hypothetical protein	-
IB024_RS01275	232174..232506	+	333	WP_187960047.1	STAS-like domain-containing protein	-
IB024_RS01280	232499..232981	+	483	WP_187960048.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	218769..247537	28768

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10724.46 Da
• Isoelectric Point: 10.2779

>T172077 WP_008505629.1 NZ_CP061088:c228268-227978 [Brucella sp. 6810]
MIEVRQTTAFTKWLNELRDTNARLRIATRIRRMELGNPGDVKAVGEGVSEMRITYGPGYRVYFVTMGSTIVVLLCGGDKS
SQSRDIAVAKQMAKEI

Nucleotide

• Length: 291 bp

>T172077 NZ_CP079186:c4857371-4857087 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10440.88 Da
• Isoelectric Point: 5.1057

>AT172077 WP_008505633.1 NZ_CP061088:c227976-227680 [Brucella sp. 6810]
MALETTRFDILDHLKTPEERLAYLEAAFEDGDPSLIAHALGDVARSVGMTAVAKEAGITREALYRSLSENGDPKLSTLLG
VTKALGIHLTVGPDKHAA

Nucleotide

• Length: 297 bp

>AT172077 NZ_CP079186:c4857603-4857361 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGTTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7L7ML11

Antitoxin

• AlphaFold DB: A0A7L7ML36