Detailed information of TA system TA172076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 136501..137171
• Replicon: chromosome
• Accession: NZ_CP061088
• Organism: Brucella sp. 6810

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IB024_RS00780
• Protein ID: WP_076770566.1
• Coordinates: 136767..137171 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D1CXH4
• Locus tag: IB024_RS00775
• Protein ID: WP_008504606.1
• Coordinates: 136501..136770 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB024_RS00755	133523..133720	+	198	WP_002963959.1	DUF3008 family protein	-
IB024_RS00760	133765..134178	-	414	WP_008935646.1	hypothetical protein	-
IB024_RS00765	134446..135417	-	972	WP_187960444.1	extensin family protein	-
IB024_RS00770	135752..136369	+	618	WP_076779584.1	30S ribosomal protein S4	-
IB024_RS00775	136501..136770	+	270	WP_008504606.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IB024_RS00780	136767..137171	+	405	WP_076770566.1	type II toxin-antitoxin system VapC family toxin	Toxin
IB024_RS00785	137470..138351	+	882	WP_002969416.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
IB024_RS00790	138338..139165	+	828	WP_187960445.1	inositol monophosphatase family protein	-
IB024_RS00795	139219..140484	-	1266	WP_187960004.1	multidrug effflux MFS transporter	-
IB024_RS00800	140753..141085	-	333	WP_009364410.1	Grx4 family monothiol glutaredoxin	-
IB024_RS00805	141267..141503	-	237	WP_008505519.1	BolA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14305.62 Da
• Isoelectric Point: 5.6357

>T172076 WP_076770566.1 NZ_CP061088:136767-137171 [Brucella sp. 6810]
MSKLYMLDTNIVSDLARNPHGPVAKHIARVGADAICVSIITACELRYGCAKKGSPRLLAQIEAILDSIEILPLDVPADTE
YGGIRAELEADGKPIGPNDFLIAAHACALGAVLVTANVGEFTRIRKLAVENWLN

Nucleotide

• Length: 405 bp

>T172076 NZ_CP079186:c4725380-4724847 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGATTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10046.62 Da
• Isoelectric Point: 7.2059

>AT172076 WP_008504606.1 NZ_CP061088:136501-136770 [Brucella sp. 6810]
MAHARRIEPTPAREAKLFRNNKSQAVRIPADFEFPGNRVLIHREGDKLIIEPVRRKNLLEVLAGLKPLGPEDAFPDIDDT
ALPLKDVDL

Nucleotide

• Length: 270 bp

>AT172076 NZ_CP079186:c4725657-4725391 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7L7MKG1