Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1279642..1280559 Replicon chromosome
Accession NZ_CP061087
Organism Bacillus velezensis strain CLA178

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A807M4S7
Locus tag IAR44_RS06540 Protein ID WP_060674890.1
Coordinates 1279813..1280559 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag IAR44_RS06535 Protein ID WP_003154807.1
Coordinates 1279642..1279812 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAR44_RS06500 1274854..1276443 + 1590 WP_053573489.1 hypothetical protein -
IAR44_RS06505 1276456..1276881 + 426 WP_053573490.1 hypothetical protein -
IAR44_RS06510 1276886..1277083 + 198 WP_012117366.1 XkdX family protein -
IAR44_RS06515 1277140..1277901 + 762 WP_060674886.1 hypothetical protein -
IAR44_RS06520 1277953..1278216 + 264 WP_031378810.1 hemolysin XhlA family protein -
IAR44_RS06525 1278230..1278493 + 264 WP_003154813.1 phage holin -
IAR44_RS06530 1278507..1279385 + 879 WP_060674888.1 N-acetylmuramoyl-L-alanine amidase -
IAR44_RS06535 1279642..1279812 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
IAR44_RS06540 1279813..1280559 - 747 WP_060674890.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
IAR44_RS06545 1280664..1281662 - 999 WP_003154805.1 inorganic phosphate transporter -
IAR44_RS06550 1281675..1282292 - 618 WP_003154804.1 DUF47 domain-containing protein -
IAR44_RS06555 1282578..1283894 - 1317 WP_007610842.1 amino acid permease -
IAR44_RS06560 1284217..1285167 + 951 WP_031378808.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29081.56 Da        Isoelectric Point: 4.7755

>T172075 WP_060674890.1 NZ_CP061087:c1280559-1279813 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGITRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMSDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T172075 NZ_CP079186:4121757-4121975 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT172075 WP_003154807.1 NZ_CP061087:c1279812-1279642 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT172075 NZ_CP079186:4121356-4121730 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A807M4S7

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References