Detailed information of TA system TA172074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 521817..522454
• Replicon: chromosome
• Accession: NZ_CP061087
• Organism: Bacillus velezensis strain CLA178

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: IAR44_RS02475
• Protein ID: WP_003156187.1
• Coordinates: 522104..522454 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: IAR44_RS02470
• Protein ID: WP_003156188.1
• Coordinates: 521817..522098 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAR44_RS02450	518182..518781	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
IAR44_RS02455	518874..519239	+	366	WP_007410229.1	holo-ACP synthase	-
IAR44_RS02460	519404..520411	+	1008	WP_077392009.1	outer membrane lipoprotein carrier protein LolA	-
IAR44_RS02465	520528..521697	+	1170	WP_015416873.1	alanine racemase	-
IAR44_RS02470	521817..522098	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
IAR44_RS02475	522104..522454	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IAR44_RS02480	522572..523393	+	822	WP_015416874.1	STAS domain-containing protein	-
IAR44_RS02485	523398..523763	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
IAR44_RS02490	523766..524167	+	402	WP_003156178.1	anti-sigma regulatory factor	-
IAR44_RS02495	524179..525186	+	1008	WP_015416875.1	PP2C family protein-serine/threonine phosphatase	-
IAR44_RS02500	525250..525579	+	330	WP_033575044.1	anti-sigma factor antagonist	-
IAR44_RS02505	525576..526058	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
IAR44_RS02510	526024..526812	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
IAR44_RS02515	526812..527414	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T172074 WP_003156187.1 NZ_CP061087:522104-522454 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T172074 NZ_CP079186:c3991822-3991505 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT172074 WP_003156188.1 NZ_CP061087:521817-522098 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT172074 NZ_CP079186:c3991512-3991225 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5