Detailed information of TA system TA172068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1532880..1533433
• Replicon: chromosome
• Accession: NZ_CP061083
• Organism: Pantoea sp. MT58

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: E0LZE2
• Locus tag: IAI47_RS07245
• Protein ID: WP_008926031.1
• Coordinates: 1533119..1533433 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: E0LZE3
• Locus tag: IAI47_RS07240
• Protein ID: WP_008926032.1
• Coordinates: 1532880..1533116 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI47_RS07220	1528629..1529360	-	732	WP_008926035.1	proteasome-type protease	-
IAI47_RS07225	1529387..1530181	-	795	WP_004571352.1	transglutaminase family protein	-
IAI47_RS07230	1530181..1531122	-	942	WP_008926034.1	alpha-E domain-containing protein	-
IAI47_RS07235	1531116..1532567	-	1452	WP_008926033.1	circularly permuted type 2 ATP-grasp protein	-
IAI47_RS07240	1532880..1533116	+	237	WP_008926032.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
IAI47_RS07245	1533119..1533433	+	315	WP_008926031.1	CcdB family protein	Toxin
IAI47_RS07255	1534123..1535244	+	1122	WP_008926030.1	Gfo/Idh/MocA family oxidoreductase	-
IAI47_RS07260	1535333..1536163	-	831	WP_008926029.1	glycosyltransferase family 8 protein	-
IAI47_RS07265	1536176..1537558	-	1383	WP_008926028.1	FAD-binding protein	-
IAI47_RS07270	1537642..1538274	-	633	WP_008926027.1	TetR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11496.44 Da
• Isoelectric Point: 8.0151

>T172068 WP_008926031.1 NZ_CP061083:1533119-1533433 [Pantoea sp. MT58]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVHALG
AVFCDASQYRTQVKAAIDFLIDGV

Nucleotide

• Length: 315 bp

>T172068 NZ_CP079181:c48956-48540 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8897.94 Da
• Isoelectric Point: 6.4612

>AT172068 WP_008926032.1 NZ_CP061083:1532880-1533116 [Pantoea sp. MT58]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTAALDAELRSHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT172068 NZ_CP079181:c49183-48953 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: E0LZE2

Antitoxin

• AlphaFold DB: E0LZE3