Detailed information of TA system TA172034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3670013..3670670
• Replicon: chromosome
• Accession: NZ_CP061080
• Organism: Mesirhizobium sp. NIBR3 strain N3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IAI54_RS17070
• Protein ID: WP_187973204.1
• Coordinates: 3670278..3670670 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IAI54_RS17065
• Protein ID: WP_187968340.1
• Coordinates: 3670013..3670273 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI54_RS17035	3665482..3666399	-	918	WP_187968335.1	3-keto-5-aminohexanoate cleavage protein	-
IAI54_RS17040	3666643..3667623	+	981	WP_187968336.1	GlxA family transcriptional regulator	-
IAI54_RS17045	3667689..3667952	+	264	WP_187968337.1	AbrB family transcriptional regulator	-
IAI54_RS17050	3667949..3668353	+	405	WP_187968338.1	type II toxin-antitoxin system VapC family toxin	-
IAI54_RS17055	3668381..3669508	-	1128	WP_187968339.1	alkene reductase	-
IAI54_RS17060	3669553..3669849	-	297	WP_210321290.1	helix-turn-helix domain-containing protein	-
IAI54_RS17065	3670013..3670273	+	261	WP_187968340.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IAI54_RS17070	3670278..3670670	+	393	WP_187973204.1	type II toxin-antitoxin system VapC family toxin	Toxin
IAI54_RS17075	3670675..3671883	-	1209	WP_187968341.1	3-oxoadipyl-CoA thiolase	-
IAI54_RS17080	3671886..3672557	-	672	WP_210321141.1	3-oxoacid CoA-transferase subunit B	-
IAI54_RS17085	3672557..3673273	-	717	WP_187968343.1	3-oxoacid CoA-transferase subunit A	-
IAI54_RS17090	3673360..3674196	+	837	WP_187968344.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• SpoVT-AbrB domain (8-58)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 13923.26 Da
• Isoelectric Point: 10.6629

>T172034 WP_187973204.1 NZ_CP061080:3670278-3670670 [Mesirhizobium sp. NIBR3]
MLDTNMVGALIGEKAARADAGLRRHRVGSVCISVVTEGEVLYGLANNPAATRKAAQMRALLSRFVILPWTSDVASVYAAV
RAAMRRNGRTMGALDMLIAAHALSMGIPLATSDRAFHHVPGLKVENWLVD

Nucleotide

• Length: 393 bp

>T172034 NZ_CP079157:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9670.64 Da
• Isoelectric Point: 4.2079

>AT172034 WP_187968340.1 NZ_CP061080:3670013-3670273 [Mesirhizobium sp. NIBR3]
MGETAKLFRNGGSQAVRLPAEFRFEGTEVNIRRDPQTGEVILSPVSPAGEPSWDDFFRLRDELDMPADFLKDRDQTQGER
DTLGDA

Nucleotide

• Length: 261 bp

>AT172034 NZ_CP079157:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA