Detailed information of TA system TA172031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2241731..2242293
• Replicon: chromosome
• Accession: NZ_CP061080
• Organism: Mesirhizobium sp. NIBR3 strain N3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IAI54_RS10395
• Protein ID: WP_187972270.1
• Coordinates: 2241925..2242293 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IAI54_RS10390
• Protein ID: WP_187972269.1
• Coordinates: 2241731..2241928 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI54_RS10370	2237428..2239275	-	1848	WP_187972265.1	ABC transporter ATP-binding protein/permease	-
IAI54_RS10375	2239806..2240255	+	450	WP_187972266.1	tRNA methyltransferase	-
IAI54_RS10380	2240266..2240526	-	261	WP_187972267.1	hypothetical protein	-
IAI54_RS10385	2240759..2241673	+	915	WP_187972268.1	oxygen-dependent coproporphyrinogen oxidase	-
IAI54_RS10390	2241731..2241928	+	198	WP_187972269.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IAI54_RS10395	2241925..2242293	+	369	WP_187972270.1	type II toxin-antitoxin system VapC family toxin	Toxin
IAI54_RS10400	2242397..2242582	+	186	WP_187972271.1	DUF1059 domain-containing protein	-
IAI54_RS10405	2242737..2244008	-	1272	WP_187972272.1	CCA tRNA nucleotidyltransferase	-
IAI54_RS10410	2244005..2244634	-	630	WP_187972273.1	CoA pyrophosphatase	-
IAI54_RS10415	2244637..2245305	-	669	WP_187973106.1	DUF1285 domain-containing protein	-
IAI54_RS10420	2245450..2246457	+	1008	WP_187972274.1	MoxR family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13781.05 Da
• Isoelectric Point: 5.8929

>T172031 WP_187972270.1 NZ_CP061080:2241925-2242293 [Mesirhizobium sp. NIBR3]
MILVDSVIWVDHIRQELPRLSRLLDNGETLCHPFVVGEIALGSLRDRQFVLESLSSLPQAPLAEHEEVLFLLEERKLFSL
GIGYVDLHLVASCLLAPGTLLWTRDRRLDAAAYHLGIKYLHG

Nucleotide

• Length: 369 bp

>T172031 NZ_CP079156:23293-23577 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7182.17 Da
• Isoelectric Point: 6.3085

>AT172031 WP_187972269.1 NZ_CP061080:2241731-2241928 [Mesirhizobium sp. NIBR3]
VRTTVTLDDVLLEQAQRFSGINERSALIEAALKALVEREAARRLAKLGGSQPDLEIPDRRRPAAE

Nucleotide

• Length: 198 bp

>AT172031 NZ_CP079156:23055-23303 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA