Detailed information of TA system TA172029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 551177..551783
• Replicon: chromosome
• Accession: NZ_CP061080
• Organism: Mesirhizobium sp. NIBR3 strain N3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IAI54_RS02545
• Protein ID: WP_187970862.1
• Coordinates: 551475..551783 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IAI54_RS02540
• Protein ID: WP_187970861.1
• Coordinates: 551177..551482 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI54_RS02510	547001..547921	+	921	WP_187972987.1	TerC family protein	-
IAI54_RS02515	548235..549032	+	798	WP_187970857.1	sel1 repeat family protein	-
IAI54_RS02520	549098..549394	-	297	WP_187970858.1	hypothetical protein	-
IAI54_RS02525	549551..549859	+	309	Protein_485	type II toxin-antitoxin system RelE/ParE family toxin	-
IAI54_RS02530	549852..550157	+	306	WP_187970859.1	putative addiction module antidote protein	-
IAI54_RS02535	550268..551056	-	789	WP_187970860.1	exodeoxyribonuclease III	-
IAI54_RS02540	551177..551482	-	306	WP_187970861.1	putative addiction module antidote protein	Antitoxin
IAI54_RS02545	551475..551783	-	309	WP_187970862.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAI54_RS02550	551926..553335	-	1410	WP_187970863.1	type I glutamate--ammonia ligase	-
IAI54_RS02555	553385..553723	-	339	WP_187970864.1	P-II family nitrogen regulator	-
IAI54_RS02560	554029..555549	+	1521	WP_187970865.1	NAD(P)H-hydrate dehydratase	-
IAI54_RS02565	555645..556637	+	993	WP_187970866.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-83)

Antitoxin

• Uncharacterized protein HI1420 (4-94)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11442.94 Da
• Isoelectric Point: 10.7140

>T172029 WP_187970862.1 NZ_CP061080:c551783-551475 [Mesirhizobium sp. NIBR3]
MLDLKQTETFRKWWMRLGDERARGVIFSRLDRLAYGHVGDVRPVGDGVSELRIHHGPGYRVYFQRRGDTIVVLLCGGDKS
TQARGTSGPRSALRRNGASEDG

Nucleotide

• Length: 309 bp

>T172029 NZ_CP079155:3557455-3557772 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10724.20 Da
• Isoelectric Point: 5.6882

>AT172029 WP_187970861.1 NZ_CP061080:c551482-551177 [Mesirhizobium sp. NIBR3]
MAEKLTTFDPAEGLTSNEAAAAFMAEAFASEDAGYIAHALGVVARATGMTRIARETGLSREQLYRSFSADGNPTLKTTLL
VMKALGIELTARSPAHRPEPL

Nucleotide

• Length: 306 bp

>AT172029 NZ_CP079155:3557765-3558052 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA