Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relE-dinJ/YafQ-RelB |
| Location | 56064..56595 | Replicon | plasmid pPAD8 |
| Accession | NZ_CP061074 | ||
| Organism | Pseudomonas aeruginosa strain PAD8 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | A0A2K4Y311 |
| Locus tag | IBG29_RS30370 | Protein ID | WP_023100672.1 |
| Coordinates | 56314..56595 (+) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | W2D9V6 |
| Locus tag | IBG29_RS30365 | Protein ID | WP_020303033.1 |
| Coordinates | 56064..56327 (+) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IBG29_RS30340 (IBG29_30610) | 51758..52075 | + | 318 | WP_171947059.1 | hypothetical protein | - |
| IBG29_RS30345 (IBG29_30615) | 52232..52729 | + | 498 | WP_023100668.1 | hypothetical protein | - |
| IBG29_RS30350 (IBG29_30620) | 52916..54274 | + | 1359 | WP_023100669.1 | replicative DNA helicase | - |
| IBG29_RS30355 (IBG29_30625) | 54274..54765 | + | 492 | WP_023100670.1 | hypothetical protein | - |
| IBG29_RS30360 (IBG29_30630) | 54997..55740 | + | 744 | WP_023100671.1 | glyoxalase superfamily protein | - |
| IBG29_RS30365 (IBG29_30635) | 56064..56327 | + | 264 | WP_020303033.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
| IBG29_RS30370 (IBG29_30640) | 56314..56595 | + | 282 | WP_023100672.1 | type II toxin-antitoxin system YafQ family toxin | Toxin |
| IBG29_RS30375 (IBG29_30645) | 56632..56967 | + | 336 | WP_023100673.1 | hypothetical protein | - |
| IBG29_RS30380 (IBG29_30650) | 57349..57801 | + | 453 | WP_023100674.1 | hypothetical protein | - |
| IBG29_RS30385 (IBG29_30655) | 57850..58572 | - | 723 | WP_031634065.1 | hypothetical protein | - |
| IBG29_RS30390 (IBG29_30660) | 58572..58922 | - | 351 | WP_023100676.1 | type II toxin-antitoxin system PemK/MazF family toxin | - |
| IBG29_RS30395 (IBG29_30665) | 58919..59167 | - | 249 | WP_023100677.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
| IBG29_RS30400 (IBG29_30670) | 59164..59583 | - | 420 | WP_041167413.1 | hypothetical protein | - |
| IBG29_RS30405 (IBG29_30675) | 60942..61472 | + | 531 | WP_145913429.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..140415 | 140415 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 10724.19 Da Isoelectric Point: 9.0277
>T172019 WP_023100672.1 NZ_CP061074:56314-56595 [Pseudomonas aeruginosa]
MRTIEQTSRFKRDYKREAKGPHRQTLASDFVAIVTALANDQPLAEKHRDHALNGDWKDHRDCHIKPDLVLIYRKPDDAVL
QLVRLGSHSELGL
MRTIEQTSRFKRDYKREAKGPHRQTLASDFVAIVTALANDQPLAEKHRDHALNGDWKDHRDCHIKPDLVLIYRKPDDAVL
QLVRLGSHSELGL
Download Length: 282 bp
>T172019 NZ_CP079151:c245571-245089 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA
Antitoxin
Download Length: 88 a.a. Molecular weight: 9471.95 Da Isoelectric Point: 4.8096
>AT172019 WP_020303033.1 NZ_CP061074:56064-56327 [Pseudomonas aeruginosa]
MSANAVVRARIDEHIKEEATVVLAAMGLTVSDAFRIMLTRVAREKALPFEPLIPNATTIEAMKEARRGGLKSFASVEDLM
ADLNADD
MSANAVVRARIDEHIKEEATVVLAAMGLTVSDAFRIMLTRVAREKALPFEPLIPNATTIEAMKEARRGGLKSFASVEDLM
ADLNADD
Download Length: 264 bp
>AT172019 NZ_CP079151:c245840-245562 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2K4Y311 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0W0N2R7 |