Detailed information of TA system TA172014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 342407..343628
• Replicon: chromosome
• Accession: NZ_CP061073
• Organism: Pseudomonas aeruginosa strain PAD8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: IBG29_RS01495
• Protein ID: WP_003102740.1
• Coordinates: 343050..343628 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IBG29_RS01490
• Protein ID: WP_003088112.1
• Coordinates: 342407..342961 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBG29_RS01465 (IBG29_02635)	337975..338526	+	552	WP_003450159.1	XRE family transcriptional regulator	-
IBG29_RS01470 (IBG29_02630)	338523..338921	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
IBG29_RS01475 (IBG29_02625)	338934..339257	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
IBG29_RS01480 (IBG29_02620)	339588..340049	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
IBG29_RS01485 (IBG29_02615)	340054..342249	+	2196	WP_014603034.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IBG29_RS01490 (IBG29_02610)	342407..342961	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
IBG29_RS01495 (IBG29_02605)	343050..343628	+	579	WP_003102740.1	HD domain-containing protein	Toxin
IBG29_RS01500 (IBG29_02600)	343737..344924	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IBG29_RS01505 (IBG29_02595)	344914..347085	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
IBG29_RS01510 (IBG29_02590)	347075..348352	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T172014 WP_003102740.1 NZ_CP061073:343050-343628 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T172014 NZ_CP079150:c4895457-4895173 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT172014 WP_003088112.1 NZ_CP061073:342407-342961 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT172014 NZ_CP079150:c4895689-4895447 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07