Detailed information of TA system TA172010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 166133..166704
• Replicon: chromosome
• Accession: NZ_CP061071
• Organism: Streptococcus mutans B04Sm5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IBL27_RS00825
• Protein ID: WP_002309274.1
• Coordinates: 166372..166704 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2J9QC51
• Locus tag: IBL27_RS00820
• Protein ID: WP_002264866.1
• Coordinates: 166133..166378 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBL27_RS00785	161641..162387	+	747	WP_002264862.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
IBL27_RS00790	162411..163271	+	861	WP_002265709.1	DegV family protein	-
IBL27_RS00795	163403..163918	+	516	WP_002267097.1	hypothetical protein	-
IBL27_RS00800	163918..164364	+	447	WP_002277323.1	hypothetical protein	-
IBL27_RS00805	164361..164555	+	195	WP_002262994.1	helix-turn-helix transcriptional regulator	-
IBL27_RS00810	164948..165394	+	447	WP_002262993.1	50S ribosomal protein L13	-
IBL27_RS00815	165419..165811	+	393	WP_002262992.1	30S ribosomal protein S9	-
IBL27_RS00820	166133..166378	+	246	WP_002264866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IBL27_RS00825	166372..166704	+	333	WP_002309274.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IBL27_RS00830	166902..167693	-	792	WP_002288766.1	DUF4037 domain-containing protein	-
IBL27_RS00835	168076..168231	+	156	WP_154079686.1	hypothetical protein	-
IBL27_RS00840	168395..169615	+	1221	WP_002309478.1	PTS sugar transporter subunit IIC	-
IBL27_RS00845	169593..170441	+	849	WP_002265112.1	alpha/beta hydrolase	-
IBL27_RS00850	170583..171185	+	603	WP_002309481.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	167860..167988	128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• Antitoxin PemI-like (1-81)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12654.52 Da
• Isoelectric Point: 6.4634

>T172010 WP_002309274.1 NZ_CP061071:166372-166704 [Streptococcus mutans B04Sm5]
MVTIKQGSIIKINLDPKQGHEQKGYRPYICLNHSIVTKYSNIAIFAPISNTKRDYPFYVSLEGTESTGKVLLDQLVTIDF
NARDYRYVEDIQEDLLDELLARVKVLFEKG

Nucleotide

• Length: 333 bp

>T172010 NZ_CP079150:c2817777-2816455 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCACCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9255.55 Da
• Isoelectric Point: 4.3021

>AT172010 WP_002264866.1 NZ_CP061071:166133-166378 [Streptococcus mutans B04Sm5]
MQLVINKWGNSSAIRLPKQLVQELQLQTNDVLDYKVSGNKIILEKVNNVPELTVEDLFKDYQGEPVNVTPVLFESVGNEQ
W

Nucleotide

• Length: 246 bp

>AT172010 NZ_CP079150:c2818043-2817777 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J9QC51