Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4524617..4525242 Replicon chromosome
Accession NZ_CP061062
Organism Klebsiella pneumoniae strain HKP0018

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IBX29_RS21690 Protein ID WP_002882817.1
Coordinates 4524617..4525000 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IBX29_RS21695 Protein ID WP_004150355.1
Coordinates 4525000..4525242 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBX29_RS21675 4521983..4522885 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IBX29_RS21680 4522882..4523517 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IBX29_RS21685 4523514..4524443 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IBX29_RS21690 4524617..4525000 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IBX29_RS21695 4525000..4525242 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IBX29_RS21700 4525447..4526364 + 918 WP_021466676.1 alpha/beta hydrolase -
IBX29_RS21705 4526378..4527319 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IBX29_RS21710 4527364..4527801 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IBX29_RS21715 4527798..4528658 - 861 WP_004146232.1 virulence factor BrkB family protein -
IBX29_RS21720 4528652..4529251 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T172005 WP_002882817.1 NZ_CP061062:c4525000-4524617 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T172005 NZ_CP079146:c245571-245089 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT172005 WP_004150355.1 NZ_CP061062:c4525242-4525000 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT172005 NZ_CP079146:c245840-245562 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References