Detailed information of TA system TA172000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1927543..1929131
• Replicon: chromosome
• Accession: NZ_CP061062
• Organism: Klebsiella pneumoniae strain HKP0018

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W8UI36
• Locus tag: IBX29_RS09280
• Protein ID: WP_002904792.1
• Coordinates: 1927543..1928865 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: IBX29_RS09285
• Protein ID: WP_002904790.1
• Coordinates: 1928865..1929131 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBX29_RS09265	1922599..1925100	+	2502	WP_032433099.1	fimbrial biogenesis outer membrane usher protein	-
IBX29_RS09270	1925088..1926092	+	1005	WP_032433097.1	type 1 fimbrial protein	-
IBX29_RS09275	1926234..1927526	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
IBX29_RS09280	1927543..1928865	-	1323	WP_002904792.1	type II toxin-antitoxin system HipA family toxin	Toxin
IBX29_RS09285	1928865..1929131	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IBX29_RS09290	1929517..1929843	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
IBX29_RS09295	1929840..1930340	-	501	WP_032433095.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
IBX29_RS09300	1930318..1931697	-	1380	WP_032433093.1	purine permease	-
IBX29_RS09305	1932043..1933197	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
IBX29_RS09310	1933178..1934101	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T172000 WP_002904792.1 NZ_CP061062:c1928865-1927543 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T172000 NZ_CP079145:c4895447-4895163 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT172000 WP_002904790.1 NZ_CP061062:c1929131-1928865 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT172000 NZ_CP079145:c4895679-4895437 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E1CEC8

Antitoxin

• AlphaFold DB: A0A0H3GXD8